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Kinetics of the appearance of Fc epsilon RI-bearing cells in
interleukin-3-dependent mouse bone marrow cultures: correlation with
histamine content and mast cell maturation
M Rottem, S Barbieri, JP Kinet and DD Metcalfe
Mast Cell Physiology Section, National Institute of Allergy and Infectious
Diseases, National Institutes of Health, Bethesda, MD 20892.
While it is known that mast cells arise from pluripotential hematopoietic
cells and express their mature phenotypes in tissues, the sequence of
events in maturation is incompletely understood. To study early mast cells,
we sorted cells from interleukin-3 (IL-3)-dependent mouse bone marrow
cultures on the basis of Fc epsilon RI and examined their morphology,
histamine content, and growth characteristics. Flow cytometric analysis and
sort showed that the Fc epsilon RI-bearing (Fc epsilon RI+) cells increased
from 0% on day 0 to 90% by day 21 and that the total number of Fc epsilon
RI+ cells increased from 0 at the start of culture to 3.75 x 10(5) cells by
day 21 from an initial population of 1 x 10(5) cells. The dissociation rate
of 125I-labeled IgE from early cultured cells resembled the dissociation
rate of mouse IgE from mature murine mast cells. Mean fluorescence
intensity increased over time, reflecting an increase in IgE receptor
density. Fc epsilon RI+ cells were also positive for Fc gamma RII/III.
Morphologic studies showed gradual acquisition of metachromatic granules in
the Fc epsilon RI+ cells, which was paralleled by an increase in histamine
content. Sorted Fc epsilon RI+ cells, when placed in liquid suspension
culture, gave rise to pure mast cell populations. Fc epsilon RI+ cells
sorted at day 3 and cultured in agarose with IL-3 gave rise to 4,800 small
and 150 medium-size mast cell colony-forming units per 10(6) cells, while
Fc epsilon RI- cells gave rise to 23 medium-size and 49 large mast cell
colony-forming units per 10(6) cells. Fc epsilon RI+ cells grown in
granulocyte-macrophage colony-stimulating factor (CSF) or macrophage- CSF
did not give rise to colony-forming units. These results show that Fc
epsilon RI+ cells have proliferative potential, but that there also is a
population of mast cell progenitor cells that have not yet expressed Fc
epsilon RI, and such individual progenitor cells have greater potential for
proliferation than cells that express Fc epsilon RI.
Volume 79,
Issue 4,
pp. 972-980,
02/15/1992
Copyright © 1992 by The American Society of Hematology
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