|
|
 Previous Article | Table of Contents | Next Article 
Stem cell factor increases colony-forming unit-spleen number in vitro in
synergy with interleukin-6, and in vivo in Sl/Sld mice as a single factor
DM Bodine, D Orlic, NC Birkett, NE Seidel and KM Zsebo
Clinical Hematology Branch NHLBI, Bethesda, MD 20892.
Hematopoiesis is thought to be modulated by interactions of progenitor
cells with hematopoietic growth factors. We have shown that colony- forming
units-spleen (CFU-S) and repopulating stem cells require interleukin-3
(IL-3) to survive in vitro, and that CFU-S number and long-term
repopulating ability can be increased by culture in the combination of IL-3
and IL-6. In this report, we describe the effects of stem cell factor (SCF)
on CFU-S and repopulating stem cells. Injection of SCF into anemic Sl/Sld
mice caused a twofold and 20-fold increase in CFU-S number in the bone
marrow and spleen of treated animals, respectively. After 6 days in
suspension culture, CFU-S number increased threefold in cultures
supplemented with SCF and IL-6, or SCF, IL-3, and IL-6 relative to the
number at day 0. The long-term repopulating ability of cells cultured in
SCF, IL-3, and IL-6 was approximately sevenfold better than that of cells
cultured in IL-3 or SCF. Similar experiments were performed on populations
of bone marrow cells enriched for, or depleted of, CFU-S by elutriation and
lineage subtraction. The combination of SCF and IL-6 increased CFU-S number
approximately fourfold to eightfold in the CFU-S-enriched fraction, but had
no effect on the CFU-S-depleted cells. These results show that SCF alone
can increase CFU-S number in vivo, and in combination with other growth
factors increases CFU-S numbers in vitro.
Volume 79,
Issue 4,
pp. 913-919,
02/15/1992
Copyright © 1992 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
R. A.J. Oostendorp, S. Gilfillan, A. Parmar, M. Schiemann, S. Marz, M. Niemeyer, S. Schill, E. Hammerschmid, V. R. Jacobs, C. Peschel, et al.
Oncostatin M-Mediated Regulation of KIT-Ligand-Induced Extracellular Signal-Regulated Kinase Signaling Maintains Hematopoietic Repopulating Activity of Lin-CD34+CD133+ Cord Blood Cells
Stem Cells,
August 1, 2008;
26(8):
2164 - 2172.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. L. Bradley, C. Couldrey, and K. D. Bunting
Hematopoietic-repopulating defects from STAT5-deficient bone marrow are not fully accounted for by loss of thrombopoietin responsiveness
Blood,
April 15, 2004;
103(8):
2965 - 2972.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. D. Bunting, H. L. Bradley, T. S. Hawley, R. Moriggl, B. P. Sorrentino, and J. N. Ihle
Reduced lymphomyeloid repopulating activity from adult bone marrow and fetal liver of mice lacking expression of STAT5
Blood,
January 15, 2002;
99(2):
479 - 487.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Gritti, P. Frolichsthal-Schoeller, R. Galli, E. A. Parati, L. Cova, S. F. Pagano, C. R. Bjornson, and A. L. Vescovi
Epidermal and Fibroblast Growth Factors Behave as Mitogenic Regulators for a Single Multipotent Stem Cell-Like Population from the Subventricular Region of the Adult Mouse Forebrain
J. Neurosci.,
May 1, 1999;
19(9):
3287 - 3297.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K.L. MacKenzie, A. Dolnikov, M. Millington, Y. Shounan, and G. Symonds
Mutant N-ras Induces Myeloproliferative Disorders and Apoptosis in Bone Marrow Repopulated Mice
Blood,
March 15, 1999;
93(6):
2043 - 2056.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. S. Pear, J. P. Miller, L. Xu, J. C. Pui, B. Soffer, R. C. Quackenbush, A. M. Pendergast, R. Bronson, J. C. Aster, M. L. Scott, et al.
Efficient and Rapid Induction of a Chronic Myelogenous Leukemia-Like Myeloproliferative Disease in Mice Receiving P210 bcr/abl-Transduced Bone Marrow
Blood,
November 15, 1998;
92(10):
3780 - 3792.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. D. Bunting, J. Galipeau, D. Topham, E. Benaim, and B. P. Sorrentino
Transduction of Murine Bone Marrow Cells With an MDR1 Vector Enables Ex Vivo Stem Cell Expansion, but These Expanded Grafts Cause a Myeloproliferative Syndrome in Transplanted Mice
Blood,
October 1, 1998;
92(7):
2269 - 2279.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Matsunaga, T. Kato, H. Miyazaki, and M. Ogawa
Thrombopoietin Promotes the Survival of Murine Hematopoietic Long-Term Reconstituting Cells: Comparison With the Effects of FLT3/FLK-2 Ligand and Interleukin-6
Blood,
July 15, 1998;
92(2):
452 - 461.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. D. Lyman and S. E. W. Jacobsen
c-kit Ligand and Flt3 Ligand: Stem/Progenitor Cell Factors With Overlapping Yet Distinct Activities
Blood,
February 15, 1998;
91(4):
1101 - 1134.
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. C. Broudy
Stem Cell Factor and Hematopoiesis
Blood,
August 15, 1997;
90(4):
1345 - 1364.
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. L. Brown, F. S. Xu, S. K. Dusing, Q. Li, R. Fischer, and M. Patchen
Serum-Free Culture Conditions for Cells Capable of Producing Long-Term Survival in Lethally Irradiated Mice
Stem Cells,
May 1, 1997;
15(3):
237 - 245.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M Ogawa, S Nishikawa, K Yoshinaga, S Hayashi, T Kunisada, J Nakao, T Kina, T Sudo, H Kodama, and S Nishikawa
Expression and function of c-Kit in fetal hemopoietic progenitor cells: transition from the early c-Kit-independent to the late c-Kit-dependent wave of hemopoiesis in the murine embryo
Development,
January 3, 1993;
117(3):
1089 - 1098.
[Abstract]
[PDF]
|
|
|
|
|
