|
|
 Previous Article | Table of Contents | Next Article 
Functional expression of the macrophage colony-stimulating factor receptor
in human THP-1 monocytic leukemia cells
R Datta, K Imamura, SJ Goldman, AC Dianoux, DW Kufe and ML Sherman
Laboratory of Clinical Pharmacology, Dana-Farber Cancer Institute, Boston,
MA 02115.
Macrophage colony-stimulating factor (M-CSF) is required for the
proliferation, differentiation, and activation of monocytes. High- affinity
receptors for M-CSF are encoded by the c-fms proto-oncogene. In the present
study, we show that c-fms transcripts are detectable in human THP-1 myeloid
leukemia cells. Furthermore, radiolabeled 125I-M- CSF is rapidly
internalized into THP-1 cells and then degraded intracellularly. The
results also show that treatment of THP-1 cells with M-CSF is associated
with the activation of protein kinase C (PKC) and the induction of tumor
necrosis factor (TNF) gene expression. TNF transcript levels were low to
undetectable in uninduced THP-1 cells, reached maximal levels by 1 hour of
exposure to M-CSF, and returned to those of control cells by 24 hours.
Transcriptional run-on analysis showed that a low level of TNF
transcription is detectable in untreated THP-1 cells, and M-CSF treatment
increased the rate of TNF transcription. Pretreatment of THP-1 cells with
pertussis toxin inhibited the increase in PKC activity but not the
induction of TNF transcripts by M-CSF. Moreover, exposure of THP-1 cells to
inhibitors of protein kinase activity blocked the increase in TNF messenger
RNA. These findings suggest that at least two M-CSF-mediated signaling
pathways exist in THP-1 cells and that the induction of TNF may be
regulated by a protein kinase-dependent mechanism distinct from PKC.
Volume 79,
Issue 4,
pp. 904-912,
02/15/1992
Copyright © 1992 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
E. Rovida, E. Spinelli, S. Sdelci, V. Barbetti, A. Morandi, S. Giuntoli, and P. Dello Sbarba
ERK5/BMK1 Is Indispensable for Optimal Colony-Stimulating Factor 1 (CSF-1)-Induced Proliferation in Macrophages in a Src-Dependent Fashion
J. Immunol.,
March 15, 2008;
180(6):
4166 - 4172.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. A. M. A. Baqui, T. F. Meiller, J. J. Chon, B.-F. Turng, and W. A. Falkler Jr.
Clin. Vaccine Immunol.,
May 1, 1998;
5(3):
341 - 347.
[Abstract]
|
|
|
|
|
|
|
A. C. Li, F. R. B. Guidez, J. G. Collier, and C. K. Glass
The Macrosialin Promoter Directs High Levels of Transcriptional Activity in Macrophages Dependent on Combinatorial Interactions between PU.1 and c-Jun
J. Biol. Chem.,
February 27, 1998;
273(9):
5389 - 5399.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. K. Tangirala, K. Murao, and O. Quehenberger
Regulation of Expression of the Human Monocyte Chemotactic Protein-1 Receptor (hCCR2) by Cytokines
J. Biol. Chem.,
March 21, 1997;
272(12):
8050 - 8056.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
