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Characterization of the myeloid-specific CD11b promoter
HL Pahl, AG Rosmarin and DG Tenen
Hematology/Oncology Division, Beth Israel Hospital, Boston, MA 02215.
The CD11b/CD18 heterodimeric surface antigen is expressed exclusively on
human monocytes, macrophages, granulocytes, and natural killer cells.
During differentiation of myeloid cell lines, CD11b steady state messenger
RNA levels increase significantly; we show here that CD11b transcription
rates increase commensurately. A 1.7-kb fragment of CD11b 5' flanking
sequence directs expression of a reporter gene specifically in myeloid cell
lines. Deletion analysis localizes elements directing high levels of
tissue-specific reporter gene expression to the 412 bp proximal to the
transcriptional start site. This sequence contains two consensus binding
sites for Sp1, a GATA motif, and a purine-rich sequence that presents
potential binding sites for members of the ets family of genes. Analysis of
this promoter should result in the isolation of myeloid-specific
transcription factors and the development of methods to direct the
myeloid-specific expression of heterologous genes.
Volume 79,
Issue 4,
pp. 865-870,
02/15/1992
Copyright © 1992 by The American Society of Hematology
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