|
|
 Previous Article | Table of Contents | Next Article 
Demonstration of a human epsilon-globin gene silencer with studies in
transgenic mice
N Raich, T Papayannopoulou, G Stamatoyannopoulos and T Enver
Department of Medicine, University of Washington, Seattle 98195.
The human epsilon-globin gene displays normal developmental regulation in
transgenic mice; it is expressed only in embryonic and in definitive
erythroid cells. We show here that deletion of a negative element located
between -182 and -467 bp upstream of the epsilon-globin gene cap site
results in continuation of epsilon gene expression in the definitive
erythroblasts of the fetal liver and in the red blood cells of adult
transgenic mice. These data provide direct in vivo evidence that cis acting
silencing elements are involved in the developmental control of the
epsilon-globin gene.
Volume 79,
Issue 4,
pp. 861-864,
02/15/1992
Copyright © 1992 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
Z. Chen, H.-Y. Luo, R. K. Basran, T.-H. Hsu, D. W. H. Mang, L. Nuntakarn, C. G. Rosenfield, G. P. Patrinos, R. C. Hardison, M. H. Steinberg, et al.
A T-to-G Transversion at Nucleotide -567 Upstream of HBG2 in a GATA-1 Binding Motif Is Associated with Elevated Hemoglobin F
Mol. Cell. Biol.,
July 1, 2008;
28(13):
4386 - 4393.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Harju-Baker, F. C. Costa, H. Fedosyuk, R. Neades, and K. R. Peterson
Silencing of A{gamma}-Globin Gene Expression during Adult Definitive Erythropoiesis Mediated by GATA-1-FOG-1-Mi2 Complex Binding at the -566 GATA Site
Mol. Cell. Biol.,
May 15, 2008;
28(10):
3101 - 3113.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. He and J. E. Russell
Dynamic posttranscriptional regulation of {epsilon}-globin gene expression in vivo
Blood,
January 15, 2007;
109(2):
795 - 801.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. A. Navas, Q. Li, K. R. Peterson, and G. Stamatoyannopoulos
Investigations of a Human Embryonic Globin Gene Silencing Element Using YAC Transgenic Mice.
Experimental Biology and Medicine,
March 1, 2006;
231(3):
328 - 334.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Wang, H. Liu, C. M. Lin, M. I. Aladjem, and E. M. Epner
Targeted Deletion of the Chicken {beta}-Globin Regulatory Elements Reveals a Cooperative Gene Silencing Activity
J. Biol. Chem.,
June 17, 2005;
280(24):
23340 - 23348.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Harju, K. J. McQueen, and K. R. Peterson
Chromatin Structure and Control of {beta}-Like Globin Gene Switching
Experimental Biology and Medicine,
October 1, 2002;
227(9):
683 - 700.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. He and J. E. Russell
A human embryonic hemoglobin inhibits Hb S polymerization in vitro and restores a normal phenotype to mouse models of sickle cell disease
PNAS,
August 6, 2002;
99(16):
10635 - 10640.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Tanimoto, Q. Liu, F. Grosveld, J. Bungert, and J. D. Engel
Context-dependent EKLF responsiveness defines the developmental specificity of the human varepsilon -globin gene in erythroid cells of YAC transgenic mice
Genes & Dev.,
November 1, 2000;
14(21):
2778 - 2794.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
J. E. Russell and S. A. Liebhaber
Reversal of Lethal alpha - and beta -Thalassemias in Mice by Expression of Human Embryonic Globins
Blood,
November 1, 1998;
92(9):
3057 - 3063.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Skarpidi, G. Vassilopoulos, G. Stamatoyannopoulos, and Q. Li
Comparison of Expression of Human Globin Genes Transferred Into Mouse Erythroleukemia Cells and in Transgenic Mice
Blood,
November 1, 1998;
92(9):
3416 - 3421.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Q. Li, C. A. Blau, C. H. Clegg, A. Rohde, and G. Stamatoyannopoulos
Multiple epsilon -Promoter Elements Participate in the Developmental Control of epsilon -Globin Genes in Transgenic Mice
J. Biol. Chem.,
July 10, 1998;
273(28):
17361 - 17367.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Li, C. T. Noguchi, W. Miller, R. Hardison, and A. N. Schechter
Multiple Regulatory Elements in the 5'-Flanking Sequence of the Human epsilon -Globin Gene
J. Biol. Chem.,
April 24, 1998;
273(17):
10202 - 10209.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. G. Marini, K. Chan, L. Casula, Y. W. Kan, A. Cao, and P. Moi
hMAF, a Small Human Transcription Factor That Heterodimerizes Specifically with Nrf1 and Nrf2
J. Biol. Chem.,
June 27, 1997;
272(26):
16490 - 16497.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Q. Li, C. Clegg, K. Peterson, S. Shaw, N. Raich, and G. Stamatoyannopoulos
Binary transgenic mouse model for studying the trans control of globin gene switching: Evidence that GATA-1 is an in vivo repressor of human varepsilon gene expression
PNAS,
March 18, 1997;
94(6):
2444 - 2448.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Q. Liu, J. Bungert, and J. D. Engel
Mutation of gene-proximal regulatory elements disrupts human varepsilon -, gamma -, and beta -globin expression in yeast artificial chromosome transgenic mice
PNAS,
January 7, 1997;
94(1):
169 - 174.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
