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Selection of benign primitive hematopoietic progenitors in chronic
myelogenous leukemia on the basis of HLA-DR antigen expression
CM Verfaillie, WJ Miller, K Boylan and PB McGlave
Department of Medicine, University of Minnesota Medical School, Minneapolis
55455.
Chronic myelogenous leukemia (CML) is a lethal malignancy of the human
hematopoietic stem cell. Here we report that coexistent benign, primitive
hematopoietic progenitors can be distinguished from their malignant
counterparts in CML bone marrow by differences in cell surface antigen
expression. Selection of bone marrow cells expressing the CD34 antigen but
lacking the HLA-DR antigen results in recovery of small lymphocyte-like
blasts, which initiate and sustain production of myeloid clonogenic progeny
in vitro. Secondary clonogenic cells derived at week 1, 5, and 8 from
long-term bone marrow cultures (LTBMCs) initiated with primitive
progenitors, which lack HLA-DR antigens, exhibit neither the Philadelphia
chromosome (Ph1) nor the corresponding bcr/abl mRNA characteristic of CML.
In contrast, clonogenic cells recovered at week 1, 5, and 8 from LTBMCs
initiated with the CML HLA- DR+ population contain Ph1 and express bcr/abl
mRNA. This observation indicates that it may be possible to select a
population of viable, exclusively benign hematopoietic stem cells from CML
bone marrow capable of repopulating the hematopoietic compartment following
autologous bone marrow transplantation.
Volume 79,
Issue 4,
pp. 1003-1010,
02/15/1992
Copyright © 1992 by The American Society of Hematology
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