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Immunovisualization of high (HK) and low (LK) molecular weight kininogens
on isolated human neutrophils
CD Figueroa, LM Henderson, J Kaufmann, RA De La Cadena, RW Colman, W Muller- Esterl and KD Bhoola
Institute of Histology and Pathology, Austral University, Valdivia, Chile.
An immunocytochemical study was performed to examine the cellular
localization and the subcellular distribution of kininogens in human blood
cells. Kininogens were visualised using the immunogold-silver staining
method and confocal scanning laser microscopy. We confirmed the existence
of high molecular weight kininogen in human neutrophils and describe for
the first time the presence of low molecular weight kininogen on these
cells. Both high and low molecular weight kininogens were restricted to the
neutrophils where they localized as clusters of immunogold particles on the
cell membrane. No labeling was observed intracellularly in organelles such
as mitochondria, endoplasmic reticulum, and azurophilic or specific
granules after permeabilization of the neutrophils with Triton X-100, a
procedure that permitted the visualization of elastase in the azurophilic
granules. Clusters of high molecular weight kininogen molecules attached to
the neutrophil surface could serve as receptors for plasma kallikrein
and/or be the source of substrate for a discrete and circumscribed
formation of kinins that may in turn facilitate the local diapedesis of
neutrophils and the transudation of plasma constituents during acute
inflammation.
Volume 79,
Issue 3,
pp. 754-759,
02/01/1992
Copyright © 1992 by The American Society of Hematology
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