A-T-rich scaffold attachment regions flank the hematopoietic serine
protease genes clustered on chromosome 14q11.2
RD Hanson and TJ Ley
Department of Medicine, Jewish Hospital, Washington University Medical
Center, St Louis, MO 63110.
We have analyzed approximately 70 kb of the chromosome 14q11.2
hematopoietic serine protease gene cluster for the presence of nuclear
scaffold attachment regions (SARs). At least 12 potential attachment sites
were identified. SARs are present on both sides of the CGL-1/CSP- B and
CGL-2/CCP-X genes and upstream from the cathepsin G (CG) gene. We have
further characterized the SARs immediately flanking the cytotoxic
lymphocyte-specific CGL-1/CSP-B gene. These 5' and 3' SARs are highly A-
T-rich, contain multiple attachment sites, and are associated with the
scaffolds of nuclei derived from both lymphoid and erythroid cell lines.
These SARs contain multiple consensus elements frequently associated with
A-T-rich sequences, including the vertebrate topoisomerase II (topo II)
consensus sequence, the A-box and T-box elements, and the yeast autonomous
replicating sequence (ARS). The potential role for the nuclear scaffold in
the transcriptional regulation of CGL-1/CSP-B expression is discussed.
Volume 79,
Issue 3,
pp. 610-618,
02/01/1992
Copyright © 1992 by The American Society of Hematology
