|
|||||||||
|
|
|
|
|
|
|
|
|
|
|
GA Denomme, JW Smith, JG Kelton and DA Bell
Department of Medicine, University Hospital, London, Ontario, Canada.
Tonsillar lymphocytes from an otherwise healthy nonthrombocytopenic male
child were fused with the lymphoblastoid cell line GM 4672. Twenty of 472
(4%) hybridomas had antiplatelet reactivity detected using intact platelets
in an enzyme-linked immunosorbent assay. One hybridoma (STO 171) reacted to
platelet glycoprotein IIb (integrin alpha IIb) as determined by
radioimmunoprecipitation and immunoblotting. Antibody specificity was
confirmed using immunodepletion experiments with isotypic antibodies
derived from a mutlitransfused Glanzmann's thrombasthenic patient. The
antibody reactivity was restricted to platelets and did not react with
other integrin alpha-chain proteins expressed on granulocytes or cultured
human brain-derived microvascular endothelial cells. These studies indicate
that lymphocytes of normal, nonthrombocytopenic individuals have the
genetic potential to produce antiplatelet autoantibodies.
This article has been cited by other articles:
| |||||||||||
 |
 |
 |
|||||||
 |
 |
 |
 |
 |
 |
 |
 |
||
| Copyright © 1992 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
