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Interleukin-11 enhances human megakaryocytopoiesis in vitro
M Teramura, S Kobayashi, S Hoshino, K Oshimi and H Mizoguchi
Department of Medicine, Tokyo Women's Medical College, Japan.
We investigated the effect of recombinant human interleukin-11 (rhIL- 11)
on human megakaryocytopoiesis. Nonadherent and T-cell-depleted human bone
marrow (BM) mononuclear cells were cultured in a serum-free agar culture
system. rhIL-11 alone did not stimulate the growth of human megakaryocyte
colonies. However, when rhIL-11 was combined with optimal or suboptimal
doses of rhIL-3, the number and size of the megakaryocyte colonies
increased. The same results were obtained when highly purified BM
CD34-positive cells were used as target cells. Next, we investigated the
effect of rhIL-11 on the ploidy of megakaryocytes. The ploidy distribution
of individual cells in megakaryocyte colonies obtained by rhIL-11 in
combination with rhIL-3 was significantly shifted towards higher values.
Furthermore, when highly purified CD41- positive BM cells were cultured in
the presence of rhIL-11, the ploidy distribution was shifted towards higher
values. This effect was not suppressed by anti-IL-6 antibody. These results
suggest that rhIL-11 acts directly as a megakaryocyte potentiator and may
play a role in regulating human megakaryocytopoiesis.
Volume 79,
Issue 2,
pp. 327-331,
01/15/1992
Copyright © 1992 by The American Society of Hematology
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