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Dexamethasone recruitment of self-renewing osteoprogenitor cells in chick
bone marrow stromal cell cultures
N Kamalia, CA McCulloch, HC Tenebaum and H Limeback
Faculty of Dentistry, University of Toronto, Ontario, Canada.
Bone marrow stromal cells are a mixed population that contribute to the
formation of the hematopoietic microenvironment. The osteogenic lineage
includes populations of cells that, in culture, form discrete nodules of
mineralized tissue when grown in the presence of ascorbic acid and sodium
beta-glycerophosphate. We have used nodule formation to assay for the
self-renewal capacity of osteoprogenitor cells in chick bone marrow
cultures. To examine the regulatory influence of dexamethasone (Dx), first
subcultures were grown continuously or split 1:1 at repeated subculture.
Cells in continuous culture exhibited less than two population doublings,
while cellular proliferation and alkaline phosphatase area were inhibited
by 10(-8) mol/L Dx. Cells in split (redistributed) cultures exhibited up to
14 population doublings and cellular proliferation was also inhibited by
Dx. In contrast with continuous cultures, redistributed cultures treated
with Dx had increased alkaline phosphatase area and 15-fold larger amounts
of mineralized tissue formation than controls. Osteogenesis was sustained
for up to four subcultures and the ratio of mineralized tissue area to
alkaline phosphotase positive cell area was at most 0.55. These data
indicate that the osteogenic lineage of bone marrow stromal cells contains
self-renewing progenitors that are recruited by Dx in culture and that at a
maximum, only 55% of the alkaline phosphatase-positive cell population
contributes to osteogenesis.
Volume 79,
Issue 2,
pp. 320-326,
01/15/1992
Copyright © 1992 by The American Society of Hematology
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