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Expression of plasma glutathione peroxidase in human liver in addition to
kidney, heart, lung, and breast in humans and rodents
FF Chu, RS Esworthy, JH Doroshow, K Doan and XF Liu
Department of Medical Oncology and Therapeutics Research, City of Hope
National Medical Center, Duarte, CA 91010-0269.
We analyzed the expression of plasma glutathione peroxidase (GSHPx-P)
messenger RNA (mRNA) in mouse, rat, and human tissues, using a human
GSHPx-P cDNA clone as the probe. Unlike the classical cellular glutathione
peroxidase (GSHPx-1), GSHPx-P expression appears to be tissue-specific. In
the mouse and rat, kidney expresses an mRNA at a high level detected with
the human probe. A signal is also detected in mRNA isolated from mouse and
rat heart, rat cardiac myocytes, mouse lung, epididymis, and the mammary
gland of midpregnant mice. No signal is detected in mRNA isolated from
mouse and rat liver, mouse brain, uterus, and testis. In human tissues, an
mRNA hybridizing to GSHPx-P cDNA is present in liver, as well as kidney,
heart, lung, breast, and placenta. We have shown that human kidney
expresses a GSHPx-P mRNA, and not a GSHPx-P-like message, by isolating a
cDNA clone from a human kidney library in lambda gt11. From the
412-nucleotide partial sequence of the kidney cDNA, which codes for the
40-170 amino acids of GSHPx-P including the TGA codon for selenocysteine,
we found complete sequence identity of the kidney cDNA with GSHPx-P
isolated from placenta. The expression of GSHPx-P mRNA in cell lines was
also studied. There is some correlation of the expression of GSHPx-P in
these cell lines with that in normal tissues. Cell lines that expressed
GSHPx-P mRNA or protein included the human hepatocarcinoma HepG2, Hep3B
cells, human kidney carcinoma A498 cells, and the human breast cancer
SK-BR-3, T47D, MDA-MB-231, and AdrrMCF-7 cells. Cell lines that did not
express GSHPx- P included human choriocarcinoma BeWo cells, human breast
cancer MCF-7, ZR-75-1, and Hs578T cells, and mouse hepatoma Hepa-1 cells.
Volume 79,
Issue 12,
pp. 3233-3238,
06/15/1992
Copyright © 1992 by The American Society of Hematology
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