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Stem cell factor stimulates the in vitro growth of bone marrow cells from
aplastic anemia patients
A Wodnar-Filipowicz, A Tichelli, KM Zsebo, B Speck and C Nissen
Department of Research, University Hospital Basel, Switzerland.
Aplastic anemia (AA) is a rare human bone marrow disorder of unknown
etiology manifested by a strongly impaired growth of hematopoietic
precursors. In this study, we examined the ability of recombinant human
stem cell factor (SCF) to stimulate proliferation in vitro of bone marrow
cells from 15 AA patients. All patients had been previously treated with
antilymphocyte globulin (ALG). SCF, in combination with erythropoietin
(Epo), interleukin-3 (IL-3), granulocyte-macrophage colony-stimulating
factor (GM-CSF), and granulocyte colony-stimulating factor (G-CSF),
increased the number of hematopoietic colonies formed in a semisolid medium
by AA marrows. Maximal colony numbers reached 30% of the numbers observed
with normal bone marrow cells. Proliferation of AA cells cultured in a
liquid medium containing SCF together with Epo, IL-3, GM-CSF, and G-CSF
approached 70% of the control level, as measured by 3H-thymidine
incorporation. The effect of the combination of SCF with the other growth
factors was more than 10 times stronger than that of the growth factors
alone. The most marked effect of SCF was on the generation of erythroid
colonies by precursor cells. The results demonstrate synergism between CSF
and other hematopoietic growth factors, resulting in the most efficient
stimulation of the in vitro growth of AA bone marrow cells described to
date. Use of SCF, either alone or in combination with other factors, may be
of potential value in treatment of AA.
Volume 79,
Issue 12,
pp. 3196-3202,
06/15/1992
Copyright © 1992 by The American Society of Hematology
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