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A PML/retinoic acid receptor alpha fusion transcript is constantly detected
by RNA-based polymerase chain reaction in acute promyelocytic leukemia
S Castaigne, N Balitrand, H de The, A Dejean, L Degos and C Chomienne
Laboratoire de Biologie Cellulaire Hematopoietique, Universite Paris VII,
France.
The t(15;17) translocation is specifically observed in patients with
promyelocytic leukemia (AML3). The chromosomal rearrangement juxtaposes the
retinoic acid receptor alpha (RAR alpha) and PML genes, resulting in
PML/RAR alpha fusion transcripts. Our previous studies have shown that a
polymerase chain reaction (PCR) amplification product could be obtained
from the cDNA of the NB4 promyelocytic cell line from which the chimaeric
PML/RAR alpha was cloned. We report here that in all 14 AML3 patients
tested, reverse transcriptase-PCR (RT-PCR) allows the detection of three
specific fusion products. In eight patients, one amplification product was
detected corresponding to the previously described abnormal fusion. Five
patients displayed a different amplified fragment corresponding to a
different fusion point. One other patient always showed a third
different-sized product. The different types of fusion transcripts
amplified were correlated to the size of the abnormal RAR alpha transcripts
detected in these patients by Northern analysis, but did not prove
determinant for either the phenotypic features or the retinoic acid
responsiveness in AML3 cells in this group of patients. The consistent
identification by RT-PCR of the fusion of the PML and RAR alpha genes in
AML3 patients suggest that this method will provide a useful tool for the
diagnosis and detection of minimal residual disease in these patients.
Volume 79,
Issue 12,
pp. 3110-3115,
06/15/1992
Copyright © 1992 by The American Society of Hematology
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