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Cellular myosin heavy chain in human leukocytes: isolation of 5' cDNA
clones, characterization of the protein, chromosomal localization, and
upregulation during myeloid differentiation
LE Toothaker, DA Gonzalez, N Tung, RS Lemons, MM Le Beau, MA Arnaout, LK Clayton and DG Tenen
Hematology/Oncology Division, Beth Israel Hospital, Boston, MA 02215.
We have isolated 5' cDNA clones encoding a member of the cellular myosin
heavy chain gene family from human leukocytes. The predicted amino acid
sequence shows 93% identity to a chicken cellular myosin heavy chain, 76%
to chicken smooth muscle, and 40% to human sarcomeric myosin heavy chain.
The mRNA is expressed as a 7.4- to 7.9-kb doublet in many nonmuscle cells,
and is upregulated in myeloid cell lines on induction from a proliferating
to a differentiated state. Antisera raised against a peptide made from the
predicted amino acid sequence specifically reacts with a 224-Kd polypeptide
in leukocyte cell lines, and the protein is also upregulated during the
induction of monocytic and granulocytic differentiation in these cells. The
gene for this cellular myosin heavy chain maps to chromosome 22, bands
q12.3-q13.1, demonstrating that it is not located in the previously
described sarcomeric gene clusters on chromosomes 14 and 17. This cellular
myosin heavy chain may be a major contractile protein responsible for
movement in myeloid cell lines because no mRNA for sarcomeric myosin heavy
chain is detected in these cells.
Volume 78,
Issue 7,
pp. 1826-1833,
10/01/1991
Copyright © 1991 by The American Society of Hematology
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