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Endothelial cells express the interleukin-1 receptor type I
D Boraschi, A Rambaldi, A Sica, P Ghiara, F Colotta, JM Wang, M de Rossi, C Zoia, G Remuzzi and F Bussolino
Sclavo Research Center, Siena, Italy.
Interleukin-1 (IL-1) profoundly affects a number of functions of vascular
cells. Two distinct IL-1 receptors (IL-1R) are expressed on different cell
types: the 80 Kd IL-1RI on T cells and fibroblasts, and the 68 Kd IL-1RII
on B cells and myelomonocytic cells. The presence and functionality of
IL-1R on vascular cells has been investigated by using polyomatransformed
mouse endothelial cell (EC) lines (sEnd.1 and tEnd.1). These cells
expressed specific and saturable binding sites for IL-1 (1,273 sites per
cell with kd 9.5 x 10(-11) mol/L for sEnd.1, and 771 sites per cell with kd
8.5 x 10(-11) mol/L for tEnd.1, with radioiodinated IL-1 alpha as ligand).
Binding of IL-1 was also evident at single cell level by autoradiography.
By cross-linking studies, the molecular weight of the IL-1 binding protein
on EC was approximately 80 Kd. This was confirmed by the presence in EC of
mRNA for the 80 Kd IL- 1RI. The IL-1RI on EC was apparently functional,
since EC responded to IL-1 with IL-6 mRNA expression and IL-6 bioactivity
production. These results were extended to human EC and vascular smooth
muscle cells, which were also found to express mRNA for IL-1RI.
Volume 78,
Issue 5,
pp. 1262-1267,
09/01/1991
Copyright © 1991 by The American Society of Hematology
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