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Activation of Epstein-Barr virus replication in Hodgkin and Reed- Sternberg
cells
G Pallesen, K Sandvej, SJ Hamilton-Dutoit, M Rowe and LS Young
Laboratory of Immunohistology, Aarhus University Hospital, Denmark.
Recent evidence has shown that Hodgkin's disease (HD) is associated with
Epstein-Barr virus (EBV) in a substantial number of cases and that in these
cases EBV DNA is localized exclusively to Hodgkin and Reed- Sternberg (RS)
cells. The virus genome is not silent in RS cells because two EBV latent
gene products, latent membrane protein (LMP) and EB early region (EBER)
transcripts, have recently been reported to be expressed in RS cells.
However, little information is available about the possible activation of
EBV replicative genes in HD. This prompted us to investigate HD biopsies
from 96 patients for expression of replicative gene products. Cryostat
sections were immunostained with monoclonal antibodies to protein BZLF1,
which controls the switch between EBV latency and replication, and also to
LMP. LMP was demonstrated in RS cells in 47 cases (49%). Three of the
LMP-positive cases (6%), but none of the LMP-negative cases, expressed the
BZLF1 protein. BZLF1 positively was confined to rare RS cells. These three
cases showed no detectable early, virus capsid, or membrane antigens. Our
findings show that activation of EBV immediate early genes occurs only
infrequently in RS cells, indicating that control of viral latency is not
severely impaired in HD patients.
Volume 78,
Issue 5,
pp. 1162-1165,
09/01/1991
Copyright © 1991 by The American Society of Hematology
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