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Neutrophils express the high affinity receptor for IgG (Fc gamma RI, CD64)
after in vivo application of recombinant human granulocyte colony-
stimulating factor
R Repp, T Valerius, A Sendler, M Gramatzki, H Iro, JR Kalden and E Platzer
Department of Medicine III, University of Erlangen-Nurnberg, Germany.
Fc receptors are important effector molecules of neutrophilic granulocytes
(polymorphonuclear neutrophils [PMN]), connecting phagocytic cells and the
specific immune response. Neutrophils from healthy donors express the
low-affinity receptors for IgG Fc gamma RII (CD32) and Fc gamma RIII
(CD16), but not the high-affinity receptor Fc gamma RI (CD64). The latter
has been found on neutrophils from patients with certain bacterial
infections and can be induced in vitro after incubation with
interferon-gamma. We show here that neutrophils strongly express Fc gamma
RI after in vivo application of recombinant human granulocyte
colony-stimulating factor (rhG-CSF). PMN from patients receiving rhG-CSF
displayed higher cytotoxicity against Daudi lymphoma cells in vitro
compared with control patients and with healthy donors. Fab fragments
against Fc gamma RII (monoclonal antibody [MoAb] IV.3) inhibited
neutrophil-mediated cytotoxicity of healthy donors but not of patients
during rhG-CSF therapy. Therefore, expression of Fc receptors by PMN was
investigated by flow cytometry and the mean fluorescence intensity (MFI)
was compared. After staining with MoAb 32.2 against Fc gamma RL, the median
MFI of neutrophils from G-CSF patients (median, 4.78; range, 2.40 to 8.50;
n = 5) was significantly higher (P = .002 and P = .001, respectively) than
the median MFI of patients not receiving G-CSF (median, 1.23; range, 1.01
to 1.58; n = 6) and the median MFI of healthy donors (median, 1.04; range,
0.67 to 1.12; n = 6). Fc gamma RI disappeared after the discontinuing of
the G- CSF injections, but was reinduced during the next treatment cycle
with rhG-CSF. The high expression of Fc gamma RI during rhG-CSF therapy
correlated with enhanced cytotoxicity. In vitro incubation with rhG-CSF
also enhances cytotoxicity, but only minor increments in Fc gamma RI
expression were observed. Thus, during in vivo application of rhG-CSF
neutrophils acquire an additional potent receptor for mediating tumor cell
killing in vitro by induction of the high-affinity receptor for IgG (Fc
gamma RI, CD64).
Volume 78,
Issue 4,
pp. 885-889,
08/15/1991
Copyright © 1991 by The American Society of Hematology
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