|
|
 Previous Article | Table of Contents | Next Article 
Membrane surface antigen expression on neutrophils: a reappraisal of the
use of surface markers for neutrophil activation
TW Kuijpers, AT Tool, CE van der Schoot, LA Ginsel, JJ Onderwater, D Roos and AJ Verhoeven
Central Laboratory of The Netherlands Red Cross Blood Transfusion Service,
Amsterdam.
Neutrophil research relies largely on studies with highly purified cells.
Yet the isolation procedures induce changes in surface expression of
several proteins. We used a large panel of monoclonal antibodies (MoAbs) to
characterize in detail the phenotypic changes during isolation and
stimulation of human neutrophils. Centrifugation on density gradients
appears to be the crucial step that causes an increase in expression of
antigens not detectable on neutrophils in whole blood samples (cytochrome
b558 recognized by MoAb 7D5; and CD10) or expressed at significantly lower
levels (CD11a, CD11b, CD11c, CD13, CD16, CD45, and CD67). Other antigens
were unaffected by the density gradient centrifugation step (CD32, CD54,
CD58, Leu-8, HLA class I). Upregulation of antigens was also determined by
stimulation of purified neutrophils. Upregulation of CD63 was an excellent
marker for release from azurophil granules. We subsequently related the
surface antigen expression to functional activities of purified
neutrophils. From these experiments, we concluded that 7D5-as "early
activation" marker--does not necessarily discriminate between primed or
resting neutrophils with respect to NADPH oxidase activity.
Volume 78,
Issue 4,
pp. 1105-1111,
08/15/1991
Copyright © 1991 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
D. Hartl, S. Krauss-Etschmann, B. Koller, P. L. Hordijk, T. W. Kuijpers, F. Hoffmann, A. Hector, E. Eber, V. Marcos, I. Bittmann, et al.
Infiltrated Neutrophils Acquire Novel Chemokine Receptor Expression and Chemokine Responsiveness in Chronic Inflammatory Lung Diseases
J. Immunol.,
December 1, 2008;
181(11):
8053 - 8067.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Drewniak, J.-J. Boelens, H. Vrielink, A. T.J. Tool, M. C.A. Bruin, M. van den Heuvel-Eibrink, L. Ball, M. D. van de Wetering, D. Roos, and T. W. Kuijpers
Granulocyte concentrates: prolonged functional capacity during storage in the presence of phenotypic changes
Haematologica,
July 1, 2008;
93(7):
1058 - 1067.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. W. Kuijpers, R. v. Bruggen, N. Kamerbeek, A. T. J. Tool, G. Hicsonmez, A. Gurgey, A. Karow, A. J. Verhoeven, K. Seeger, O. Sanal, et al.
Natural history and early diagnosis of LAD-1/variant syndrome
Blood,
April 15, 2007;
109(8):
3529 - 3537.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. A. C. van Helvoort, Y. F. Heijdra, R. C. C. de Boer, A. Swinkels, H. M. H. Thijs, and P. N. R. Dekhuijzen
Six-Minute Walking-Induced Systemic Inflammation and Oxidative Stress in Muscle-Wasted COPD Patients
Chest,
February 1, 2007;
131(2):
439 - 445.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Mollinedo, J. Calafat, H. Janssen, B. Martin-Martin, J. Canchado, S. M. Nabokina, and C. Gajate
Combinatorial SNARE Complexes Modulate the Secretion of Cytoplasmic Granules in Human Neutrophils.
J. Immunol.,
September 1, 2006;
177(5):
2831 - 2841.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. van Mirre, W. B. Breunis, J. Geissler, C. E. Hack, M. de Boer, D. Roos, and T. W. Kuijpers
Neutrophil responsiveness to IgG, as determined by fixed ratios of mRNA levels for activating and inhibitory Fc{gamma}RII (CD32), is stable over time and unaffected by cytokines
Blood,
July 15, 2006;
108(2):
584 - 590.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. A. C. van Helvoort, Y. F. Heijdra, L. M. A. Heunks, P. L. M. Meijer, W. Ruitenbeek, H. M. H. Thijs, and P. N. R. Dekhuijzen
Supplemental Oxygen Prevents Exercise-induced Oxidative Stress in Muscle-wasted Patients with Chronic Obstructive Pulmonary Disease
Am. J. Respir. Crit. Care Med.,
May 15, 2006;
173(10):
1122 - 1129.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Wehlin, M. Lofdahl, J. Lundahl, and M. Skold
Reduced Intracellular Oxygen Radical Production in Whole Blood Leukocytes From COPD Patients and Asymptomatic Smokers
Chest,
October 1, 2005;
128(4):
2051 - 2058.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. C. W. Poland, J.-J. G. Vallejo, H. W. M. Niessen, R. Nijmeyer, J. Calafat, C. E. Hack, B. Van het Hof, and W. Van Dijk
Activated human PMN synthesize and release a strongly fucosylated glycoform of {alpha}1-acid glycoprotein, which is transiently deposited in human myocardial infarction
J. Leukoc. Biol.,
August 1, 2005;
78(2):
453 - 461.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Verploegen, L. Ulfman, H. W. M. van Deutekom, C. van Aalst, H. Honing, J.-W. J. Lammers, L. Koenderman, and P. J. Coffer
Characterization of the role of CaMKI-like kinase (CKLiK) in human granulocyte function
Blood,
August 1, 2005;
106(3):
1076 - 1083.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. L. Rosales, J. D. Ernst, J. Hallows, and K.-Y. Lee
GTP-dependent Secretion from Neutrophils Is Regulated by Cdk5
J. Biol. Chem.,
December 24, 2004;
279(52):
53932 - 53936.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. L. Smith, M. Sperandio, E. V. Galkina, and K. Ley
Autoperfused mouse flow chamber reveals synergistic neutrophil accumulation through P-selectin and E-selectin
J. Leukoc. Biol.,
November 1, 2004;
76(5):
985 - 993.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. T. Elghetany and F. Lacombe
Physiologic variations in granulocytic surface antigen expression: impact of age, gender, pregnancy, race, and stress
J. Leukoc. Biol.,
February 1, 2004;
75(2):
157 - 162.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Tanaka, J. C. Edberg, W. Chatham, G. Fassina, and R. P. Kimberly
Fc{gamma}RIIIb Allele-Sensitive Release of {alpha}-Defensins: Anti-Neutrophil Cytoplasmic Antibody-Induced Release of Chemotaxins
J. Immunol.,
December 1, 2003;
171(11):
6090 - 6096.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Brickson, L. L. Ji, K. Schell, R. Olabisi, B. St. Pierre Schneider, and T. M. Best
M1/70 attenuates blood-borne neutrophil oxidants, activation, and myofiber damage following stretch injury
J Appl Physiol,
September 1, 2003;
95(3):
969 - 976.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Mattsson, T. Persson, P. Andersson, J. Rollof, and A. Egesten
Peptidoglycan Induces Mobilization of the Surface Marker for Activation Marker CD66b in Human Neutrophils but Not in Eosinophils
Clin. Vaccine Immunol.,
May 1, 2003;
10(3):
485 - 488.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Mollinedo, B. Martin-Martin, J. Calafat, S. M. Nabokina, and P. A. Lazo
Role of Vesicle-Associated Membrane Protein-2, Through Q-Soluble N-Ethylmaleimide-Sensitive Factor Attachment Protein Receptor/R-Soluble N-Ethylmaleimide-Sensitive Factor Attachment Protein Receptor Interaction, in the Exocytosis of Specific and Tertiary Granules of Human Neutrophils
J. Immunol.,
January 15, 2003;
170(2):
1034 - 1042.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Zahler, A. Hoffmann, T. Gloe, and U. Pohl
Gap-junctional coupling between neutrophils and endothelial cells: a novel modulator of transendothelial migration
J. Leukoc. Biol.,
January 1, 2003;
73(1):
118 - 126.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. J. Wanten, M. G. Netea, T. H. Naber, J. H. Curfs, L. E. Jacobs, T. J. Verver-Jansen, and B.-J. Kullberg
Parenteral Administration of Medium- but Not Long-Chain Lipid Emulsions May Increase the Risk for Infections by Candida albicans
Infect. Immun.,
November 1, 2002;
70(11):
6471 - 6474.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Feuk-Lagerstedt, M. Samuelsson, W. Mosgoeller, C. Movitz, A. Rosqvist, J. Bergstrom, T. Larsson, M. Steiner, R. Prohaska, and A. Karlsson
The presence of stomatin in detergent-insoluble domains of neutrophil granule membranes
J. Leukoc. Biol.,
November 1, 2002;
72(5):
970 - 977.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. A. Rarok, C. A. Stegeman, P. C. Limburg, and C. G. M. Kallenberg
Neutrophil Membrane Expression of Proteinase 3 (PR3) Is Related to Relapse in PR3-ANCA-Associated Vasculitis
J. Am. Soc. Nephrol.,
September 1, 2002;
13(9):
2232 - 2238.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. J. Kwakkenbos, G.-W. Chang, H.-H. Lin, W. Pouwels, E. C. de Jong, R. A. W. van Lier, S. Gordon, and J. Hamann
The human EGF-TM7 family member EMR2 is a heterodimeric receptor expressed on myeloid cells
J. Leukoc. Biol.,
May 1, 2002;
71(5):
854 - 862.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Wanten, A. Rops, S. E. van Emst-de Vries, T. Naber, and P. H. G. M. Willems
Prompt inhibition of fMLP-induced Ca2+ mobilization by parenteral lipid emulsions in human neutrophils
J. Lipid Res.,
April 1, 2002;
43(4):
550 - 556.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Laufs, K. Muller, J. Fleischer, N. Reiling, N. Jahnke, J. C. Jensenius, W. Solbach, and T. Laskay
Intracellular Survival of Leishmania major in Neutrophil Granulocytes after Uptake in the Absence of Heat-Labile Serum Factors
Infect. Immun.,
February 1, 2002;
70(2):
826 - 835.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Durand, Y. Renaudineau, J.-O. Pers, P. Youinou, and C. Jamin
Cross-Linking of Human Fc{gamma}RIIIb Induces the Production of Granulocyte Colony-Stimulating Factor and Granulocyte-Macrophage Colony-Stimulating Factor by Polymorphonuclear Neutrophils
J. Immunol.,
October 1, 2001;
167(7):
3996 - 4007.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. J. Anderson, W. T. Roswit, M. J. Holtzman, J. C. Hogg, and S. F. Van Eeden
Effect of mechanical deformation of neutrophils on their CD18/ICAM-1-dependent adhesion
J Appl Physiol,
September 1, 2001;
91(3):
1084 - 1090.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. J. Cotter, K. E. Norman, P. G. Hellewell, and V. C. Ridger
A Novel Method for Isolation of Neutrophils from Murine Blood Using Negative Immunomagnetic Separation
Am. J. Pathol.,
August 1, 2001;
159(2):
473 - 481.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
G. F. Kormoczi, U. M. Wolfel, A. R. Rosenkranz, W. H. Horl, R. Oberbauer, and G. J. Zlabinger
Serum Proteins Modified by Neutrophil-Derived Oxidants as Mediators of Neutrophil Stimulation
J. Immunol.,
July 1, 2001;
167(1):
451 - 460.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M.A Costa, L.E.A de Wit, V de Valk, P Serrano, A.J Wardeh, P.W Serruys, and W Sluiter
Indirect evidence for a role of a subpopulation of activated neutrophils in the remodelling process after percutaneous coronary intervention
Eur. Heart J.,
April 1, 2001;
22(7):
580 - 586.
[Abstract]
[PDF]
|
|
|
|
|
|
|
J. M. van den Berg, S. Weyer, J. J. Weening, D. Roos, and T. W. Kuijpers
Divergent effects of tumor necrosis factor {alpha} on apoptosis of human neutrophils
J. Leukoc. Biol.,
March 1, 2001;
69(3):
467 - 473.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
C. Rubel, G. C. Fernandez, G. Dran, M. B. Bompadre, M. A. Isturiz, and M. S. Palermo
Fibrinogen Promotes Neutrophil Activation and Delays Apoptosis
J. Immunol.,
February 1, 2001;
166(3):
2002 - 2010.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Falanga, M. Marchetti, V. Evangelista, A. Vignoli, M. Licini, M. Balicco, S. Manarini, G. Finazzi, C. Cerletti, and T. Barbui
Polymorphonuclear leukocyte activation and hemostasis in patients with essential thrombocythemia and polycythemia vera
Blood,
December 15, 2000;
96(13):
4261 - 4266.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Martin-Martin, S. M. Nabokina, J. Blasi, P. A. Lazo, and F. Mollinedo
Involvement of SNAP-23 and syntaxin 6 in human neutrophil exocytosis
Blood,
October 1, 2000;
96(7):
2574 - 2583.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Koenderman, D. Kanters, B. Maesen, J. Raaijmakers, J.-W. J. Lammers, J. de Kruif, and T. Logtenberg
Monitoring of neutrophil priming in whole blood by antibodies isolated from a synthetic phage antibody library
J. Leukoc. Biol.,
July 1, 2000;
68(1):
58 - 64.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
C. Dahlgren, T. Christophe, F. Boulay, P. N. Madianos, M. J. Rabiet, and A. Karlsson
The synthetic chemoattractant Trp-Lys-Tyr-Met-Val-DMet activates neutrophils preferentially through the lipoxin A4 receptor
Blood,
March 1, 2000;
95(5):
1810 - 1818.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. J van Tits, P. N Demacker, J. de Graaf, H. L Hak-Lemmers, and A. F Stalenhoef
{alpha}-Tocopherol supplementation decreases production of superoxide and cytokines by leukocytes ex vivo in both normolipidemic and hypertriglyceridemic individuals1
Am. J. Clinical Nutrition,
February 1, 2000;
71(2):
458 - 464.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Hallden, M. Hadi, H. T. Hong, and G. W. Aponte
Y Receptor-mediated Induction of CD63 Transcripts, a Tetraspanin Determined To Be Necessary for Differentiation of the Intestinal Epithelial Cell Line, hBRIE 380i Cells
J. Biol. Chem.,
September 24, 1999;
274(39):
27914 - 27924.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Szekely, B. Heindl, S. Zahler, P. F. Conzen, and B. F. Becker
S(+)-Ketamine, but Not R(-)-Ketamine, Reduces Postischemic Adherence of Neutrophils in the Coronary System of Isolated Guinea Pig Hearts
Anesth. Analg.,
May 1, 1999;
88(5):
1017 - 1024.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. C M. Kobold, C. G M Kallenberg, and J. W. C. Tervaert
Monocyte activation in patients with Wegener's granulomatosis
Ann Rheum Dis,
April 1, 1999;
58(4):
237 - 245.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
N. Fujisawa, S. Hayashi, A. Kurdowska, J. M. Noble, K. Naitoh, and E. J. Miller
Staphylococcal Enterotoxin A-Induced Injury of Human Lung Endothelial Cells and IL-8 Accumulation Are Mediated by TNF-{alpha}
J. Immunol.,
November 15, 1998;
161(10):
5627 - 5632.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Itoh, K. Okubo, H. Utiyama, T. Hirano, J. Yoshii, and K. Matsubara
Expression Profile of Active Genes in Granulocytes
Blood,
August 15, 1998;
92(4):
1432 - 1441.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Legras, U. Gunthert, R. Stauder, F. Curt, S. Oliferenko, H.C. Kluin-Nelemans, J.P. Marie, S. Proctor, C. Jasmin, and F. Smadja-Joffe
A Strong Expression of CD44-6v Correlates With Shorter Survival of Patients With Acute Myeloid Leukemia
Blood,
May 1, 1998;
91(9):
3401 - 3413.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
