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Specific binding sites for H-ferritin on human lymphocytes: modulation
during cellular proliferation and potential implication in cell growth
control
S Fargion, AL Fracanzani, B Brando, P Arosio, S Levi and G Fiorelli
Institute of Internal Medicine, Milan, Italy.
Interactions between human recombinant H- and L-ferritins and human
lymphocytes were studied in vitro by direct binding assays and by flow
cytometry. L-ferritin did not cause detectable specific binding, whereas
H-ferritin showed a specific and saturable binding that increased markedly
in phytohemagglutinin (PHA)-stimulated cells. This ferritin bound up to 30%
of CD4+ and CD8+ T-lymphocytes and most B cells, indicating that expression
of ferritin binding sites is not related to cell lineage or function.
Dual-color flow cytometry experiments showed that ferritin binding sites
were present on cells expressing the proliferation markers HLA-DR, MLR3,
interleukin 2 (IL- 2), and transferrin receptors (Tf-R). In addition, after
PHA induction, the time course of the expression of H-ferritin binding
sites was similar to those of the above proliferation markers. Ferritin
binding sites were observed in lymphocytes at all cell cycle phases,
including the early S-phase. H-Ferritin at nanomolar and picomolar
concentrations had an inhibitory effect on PHA-induced blastogenesis. We
propose that H-ferritin binding sites behave like proliferation markers,
with the unusual function of downregulating proliferation.
Volume 78,
Issue 4,
pp. 1056-1061,
08/15/1991
Copyright © 1991 by The American Society of Hematology
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