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Identification and characterization of a low-affinity granulocyte-
macrophage colony-stimulating factor receptor on primary and cultured human
melanoma cells
GC Baldwin, DW Golde, GF Widhopf, J Economou and JC Gasson
Department of Medicine, Jonsson Comprehensive Cancer Center, Los Angeles,
CA.
Hematopoietic growth factor receptors are present on cells of normal
nonhematopoietic tissues such as endothelium and placenta. We previously
demonstrated functional human granulocyte-macrophage colony- stimulating
factor (GM-CSF) receptors on small cell carcinoma of the lung cell lines,
and others have reported that certain solid tumor cell lines respond to
GM-CSF in clonogenic assays. In the current study, we examine human
melanoma cell lines and fresh specimens of melanoma to determine whether
they have functional GM-CSF receptors. Scatchard analyses of 125I-GM-CSF
equilibrium binding to melanoma cell lines showed a mean of 542 +/- 67
sites per cell with a kd of 0.72 +/- 0.14 nmol/L. Cross-linking studies in
the melanoma cell line, M14, showed a major GM-CSF receptor species of
84,000 daltons. Under the conditions tested, the M14 cells did not have a
proliferative response to GM-CSF in vitro, nor was any induction of primary
response genes detected by Northern analysis in response to GM-CSF. Studies
to determine internal translocation of the receptor-ligand complex
indicated less than 10% of the 125I-GM-CSF internalized was specifically
bound to receptors. Primary melanoma cells from five surgical specimens had
GM-CSF receptors; Scatchard analysis was performed on one sample, showing
555 sites/cell with a kd of 0.23 nmol/L. These results indicate that human
tumor cells may express a low-affinity GM-CSF receptor protein that
localizes to the cell surface and binds ligand, but lacks functional
components or accessory factors needed to transduce a signal.
Volume 78,
Issue 3,
pp. 609-615,
08/01/1991
Copyright © 1991 by The American Society of Hematology
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