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Establishment and characterization of a new human leukemia cell line
derived from M4E0
K Yanagisawa, T Horiuchi and S Fujita
First Department of Internal Medicine, School of Medicine, Ehime
University, Japan.
A new human leukemia cell line, designated as ME-1, was established from
the peripheral blood leukemia cells of a patient with acute myelomonocytic
leukemia with eosinophilia (M4E0). This cell line has the characteristic
chromosome abnormality of M4E0, inv(16) (p13q22). When cultured in RPMI
1640 medium containing 10% fetal calf serum, ME-1 cells were monoblastoid,
but with the addition of cytokines such as interleukin-3 (IL-3),
granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-4, or medium
conditioned by phytohemagglutinin-stimulated human peripheral leukocytes
(PHA-LCM), the cells exhibited differentiation to macrophage-like cells.
PHA-LCM also promoted eosinophilic-lineage differentiation of this cell
line, although IL-5 did not do so. To elucidate the mechanism of
proliferation and differentiation of ME-1 cells, we studied the effect of a
potent inhibitor of protein kinase C, 1-(5-isoquinolinyl-sulfonyl)-2-
methylpiperazine (H-7), on colony formation of ME-1 cells. H-7 inhibited
colony formation of ME-1 cells by IL-3 or GM-CSF dose dependently, but had
little inhibitory effect on colony formation by IL- 4. These results
indicate that the proliferation and differentiation of ME-1 cells by IL-3
or GM-CSF were related to the activation of protein kinase C, while those
by IL-4 involved other regulatory systems. ME-1 cells should be useful for
studying the pathogenesis of M4E0 and the mechanisms of proliferation and
differentiation of leukemic and normal progenitors by cytokines.
Volume 78,
Issue 2,
pp. 451-457,
07/15/1991
Copyright © 1991 by The American Society of Hematology
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