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Characterization of a receptor for interleukin-5 on human eosinophils and
the myeloid leukemia line HL-60
E Ingley and IG Young
Medical Molecular Biology Group, John Curtin School of Medical Research,
Australian National University, Canberra.
Interleukin-5 (IL-5) promotes the growth and differentiation of human
eosinophils and may regulate the selective eosinophilia and eosinophil
activation seen in certain diseases. Radiolabeled recombinant human IL- 5
(hIL-5) was used to characterize the IL-5 receptor present on normal human
eosinophils and on the myeloid leukemia line HL-60, which can be induced to
differentiate into eosinophilic cells. Binding studies with eosinophils and
HL-60 cells grown under alkaline conditions demonstrated similar
high-affinity binding sites for hIL-5 on both cell types with kd values of
approximately 400 pmol/L. The binding observed was specific in that it was
not inhibited by hIL-3, human granulocyte- macrophage colony-stimulating
factor, or hIL-2. Binding studies with a number of other human cell lines,
including a B-lymphoma line, and with lymphocyte and neutrophil
preparations were also performed, but IL-5 receptors were not detectable on
these cells. The number of hIL-5 receptors on HL-60 cells could be
correlated with its propensity to differentiate towards an eosinophilic
cell type. Expression of hIL-5 receptors on HL-60 cells was upregulated by
butyric acid under alkaline conditions, downregulated by hIL-3, virtually
eliminated by dimethyl sulfoxide and hIL-5, while hIL-2 had no detectable
effect. One major 125I-hIL-5-crosslinked complex of 75 to 85 Kd in Mr was
detected on HL- 60 cells using crosslinking agents giving a molecular mass
of 55 to 60 Kd for the hIL-5 receptor itself. Studies using cellular
autoradiography showed that IL-5 receptors were evenly distributed on
eosinophils but that receptor distribution on HL-60 cells was noticeably
heterogeneous. Eosinophils were the only cells in slides prepared from
peripheral blood that had detectable levels of IL-5 receptors in agreement
with the specific action of IL-5 on the human eosinophil lineage.
Volume 78,
Issue 2,
pp. 339-344,
07/15/1991
Copyright © 1991 by The American Society of Hematology
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