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Expression of alpha-smooth muscle actin in murine bone marrow stromal cells
A Peled, D Zipori, O Abramsky, H Ovadia and E Shezen
Department of Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
Human fibrotic bone marrow (BM) stroma has been shown to contain alpha-
smooth muscle actin (alpha-SMA)-positive cells. These closely resemble
myofibroblasts that were described in other fibrotic tissues. We studied
the expression of alpha-SMA in a series of murine BM-derived stromal cell
lines to investigate the cellular origin and functional significance of
myofibroblast-like cells in hematopoietic tissues. Although these cell
lines differed in their biologic properties, most of them expressed
alpha-SMA under certain conditions. Cells expressing alpha-SMA constituted
a minor population in post-confluent, growth- arrested cultures. However,
the incidence of cells expressing alpha-SMA increased significantly when
cultures were transferred to nonconfluent conditions. A similar increase in
alpha-SMA-positive cells occurred after a strip of cells was scraped away
from the confluent cell layer; the cells of the affected area acquired
alpha-SMA-positive contractile phenotype. The relationship between
alpha-SMA expression and hematopoietic activity was studied using a cloned
cell line of BM origin (14F1.1). The ability of these endothelial-adipocyte
cells to support hematopoiesis in vitro was maximal under confluent
conditions, whereas their expression of alpha-SMA under such conditions was
residual. Moreover, in long-term BM cultures supported by confluent 14F1.1
cells, stromal areas associated with proliferating hematopoietic
precursors, known as "cobblestone areas," were devoid of alpha-SMA-
positive cells. These observations suggest that the expression of alpha-
SMA is reversible and inversely related to hematopoietic activity.
Volume 78,
Issue 2,
pp. 304-309,
07/15/1991
Copyright © 1991 by The American Society of Hematology
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