|
|
 Previous Article | Table of Contents | Next Article 
Establishment in culture and characterization of a strain with mast cell
and monocytic properties from the bone marrow of a child with diffuse
cutaneous mastocytosis
SA Krilis, SG Warneford, J Macpherson, S Kyradji, L Dalla-Pozza, A Kemp, R Mitchell, CN Chesterman, PB Rowe and G Symonds
School of Medicine, University of New South Wales, Kogarah, Australia.
Bone marrow was isolated from a child with congenital mastocytosis. Upon
prolonged in vitro culture, initially in the presence of interleukin-3
(IL-3), a population of relatively large fusiform, strongly adherent cells
grew out plus a subpopulation of smaller nonadherent cells. The morphology
of the adherent cells was not typical of fibroblasts, epithelial cells, nor
of standard hematopoietic cell types, whereas the morphology of the
nonadherent cells resembled mast cells. Neither cell type required the
presence of IL-3 nor a feeder layer of fibroblasts for continued growth.
Attempts to isolate the two populations were unsuccessful. This cell strain
comprised of both cell populations has been termed human bone
marrow-derived mastocytosis cells (HBM-M). These cells were found to
possess some of the cytochemical, ultrastructural, and surface phenotypic
features of degranulated mast cells. They reacted with the mast cell
marker, monoclonal antibody YB5.B8, but not with the basophil specific
monoclonal antibody Bsp-1 and released the inflammatory mediators
histamine, leukotriene C4, prostaglandin D2, and platelet-activating factor
constitutively. This release was not potentiated by immunologic- or
nonimmunologic-activating stimuli. In addition, they exhibited cytochemical
and surface phenotypic features of monocytes. Our results indicate that a
population of abnormal proliferative cells exist in the marrow of this
patient; that these cells may be responsible for the patient's pronounced
systemic proliferation of mast cells and the associated symptoms; and that
the cell's mast cell, monocyte properties may be indicative of a common
bone marrow-derived mast cell/monocyte precursor.
Volume 78,
Issue 2,
pp. 290-303,
07/15/1991
Copyright © 1991 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
J. C. Qi, R. L. Stevens, R. Wadley, A. Collins, M. Cooley, H. M. Naif, N. Nasr, A. Cunningham, G. Katsoulotos, Y. Wanigasek, et al.
IL-16 Regulation of Human Mast Cells/Basophils and Their Susceptibility to HIV-1
J. Immunol.,
April 15, 2002;
168(8):
4127 - 4134.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Li, J. J. Macpherson, S. Adelstein, C. L. Bunn, K. Atkinson, K. Phadke, and S. A. Krilis
Conditioned Media from a Cell Strain Derived from a Patient with Mastocytosis Induces Preferential Development of Cells That Possess High Affinity IgE Receptors and the Granule Protease Phenotype of Mature Cutaneous Mast Cells
J. Biol. Chem.,
February 3, 1995;
270(5):
2258 - 2263.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
