|
|
 Previous Article | Table of Contents | Next Article 
All advanced stage non-Hodgkin's lymphomas with a polymerase chain reaction
amplifiable breakpoint of bcl-2 have residual cells containing the bcl-2
rearrangement at evaluation and after treatment
JG Gribben, As Freedman, SD Woo, K Blake, RS Shu, G Freeman, JA Longtine, GS Pinkus and LM Nadler
Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, MA
02115.
Polymerase chain reaction (PCR) of bcl-2 provides an extremely sensitive
method to detect minimal disease in approximately 50% of patients with
non-Hodgkin's lymphomas (NHL). In an attempt to determine the clinical
usefulness of this technique, we examined the bone marrow (BM) of 152
patients with advanced-stage NHL at the time of evaluation and after
induction or salvage chemotherapy before autologous BM transplantation. The
BM proved to be an accessible and reproducible tissue source to determine
PCR positivity because all of the 102 patients examined had the same
PCR-amplifiable breakpoint in their BM and lymph node. At the time of
evaluation, PCR analysis in advanced- stage NHL patients added little
additional information to morphologic analysis because each technique
identified BM infiltration in approximately 70% of patients. PCR was
significantly more useful in determining BM infiltration after induction or
salvage therapy. At that time, approximately 50% of patients had
morphologically normal BM, whereas PCR analysis remained positive in 100%
of those with an amplifiable breakpoint. These observations were confirmed
in a clinical trial attempting to induce remission in previously untreated
low-grade advanced-stage NHL patients. In this series, PCR was positive in
all patients after treatment although the BM was histologically uninvolved
in 50% of cases, showing that conventional therapy did not eradicate
bcl-2-positive cells.
Volume 78,
Issue 12,
pp. 3275-3280,
12/15/1991
Copyright © 1991 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
P. Ruminy, F. Jardin, J.-M. Picquenot, F. Parmentier, N. Contentin, G. Buchonnet, S. Tison, V. Rainville, H. Tilly, and C. Bastard
S{micro} mutation patterns suggest different progression pathways in follicular lymphoma: early direct or late from FL progenitor cells
Blood,
September 1, 2008;
112(5):
1951 - 1959.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Montoto, C. Moreno, E. Domingo-Domenech, C. Estany, A. Oriol, A. Altes, J. Besalduch, C. Pedro, S. Gardella, L. Escoda, et al.
High clinical and molecular response rates with fludarabine, cyclophosphamide and mitoxantrone in previously untreated patients with advanced stage follicular lymphoma
Haematologica,
February 1, 2008;
93(2):
207 - 214.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Witzens-Harig, C. Heilmann, M. Hensel, M. Kornacker, A. Benner, R. Haas, S. Fruehauf, and A. D. Ho
Long-Term Follow-Up of Patients with Non-Hodgkin Lymphoma Following Myeloablative Therapy and Autologous Transplantation of CD34+-Selected Peripheral Blood Progenitor Cells
Stem Cells,
January 1, 2007;
25(1):
228 - 235.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Q. Liu, L. Fayad, F. Cabanillas, F. B. Hagemeister, G. D. Ayers, M. Hess, J. Romaguera, M. A. Rodriguez, A. M. Tsimberidou, S. Verstovsek, et al.
Improvement of Overall and Failure-Free Survival in Stage IV Follicular Lymphoma: 25 Years of Treatment Experience at The University of Texas M.D. Anderson Cancer Center
J. Clin. Oncol.,
April 1, 2006;
24(10):
1582 - 1589.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Pennell, A. Woods, M. Reis, R. Buckstein, D. Spaner, K. Imrie, K. Hewitt, A. Boudreau, A. Seth, and N. L. Berinstein
Association of Clinical Status of Follicular Lymphoma Patients after Autologous Stem Cell Transplant and Quantitative Assessment of Lymphoma in Blood and Bone Marrow as Measured by SYBR Green I Polymerase Chain Reaction
J. Mol. Diagn.,
February 1, 2006;
8(1):
40 - 50.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A K H Shia, G-G Gan, S Jairaman, and S-C Peh
High frequency of germinal centre derivation in diffuse large B cell lymphoma from Asian patients
J. Clin. Pathol.,
September 1, 2005;
58(9):
962 - 967.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Schnell, M. Dietlein, J. O. Staak, P. Borchmann, K. Schomaecker, T. Fischer, W. Eschner, H. Hansen, F. Morschhauser, H. Schicha, et al.
Treatment of Refractory Hodgkin's Lymphoma Patients With an Iodine-131-Labeled Murine Anti-CD30 Monoclonal Antibody
J. Clin. Oncol.,
July 20, 2005;
23(21):
4669 - 4678.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Vallisa, P. Bernuzzi, L. Arcaini, S. Sacchi, V. Callea, R. Marasca, A. Lazzaro, E. Trabacchi, E. Anselmi, A. L. Arcari, et al.
Role of Anti-Hepatitis C Virus (HCV) Treatment in HCV-Related, Low-Grade, B-Cell, Non-Hodgkin's Lymphoma: A Multicenter Italian Experience
J. Clin. Oncol.,
January 20, 2005;
23(3):
468 - 473.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Zompi, L.-J. Couderc, J. Cadranel, M. Antoine, B. Epardeau, J. Fleury-Feith, N. Popa, F. Santoli, J.-P. Farcet, and M.-H. Delfau-Larue
Clonality analysis of alveolar B lymphocytes contributes to the diagnostic strategy in clinical suspicion of pulmonary lymphoma
Blood,
April 15, 2004;
103(8):
3208 - 3215.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Ladetto, D. Drandi, M. Compagno, M. Astolfi, F. Volpato, C. Voena, A. Novarino, B. Pollio, A. Addeo, I. Ricca, et al.
PCR-Detectable Nonneoplastic Bcl-2/IgH Rearrangements Are Common in Normal Subjects and Cancer Patients at Diagnosis but Rare in Subjects Treated With Chemotherapy
J. Clin. Oncol.,
April 1, 2003;
21(7):
1398 - 1403.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. A. Peterson, G. R. Petroni, G. Frizzera, M. Barcos, C. D. Bloomfield, N. I. Nissen, D. D. Hurd, E. S. Henderson, G. P. Sartiano, J. L. Johnson, et al.
Prolonged Single-Agent Versus Combination Chemotherapy in Indolent Follicular Lymphomas: A Study of the Cancer and Leukemia Group B
J. Clin. Oncol.,
January 1, 2003;
21(1):
5 - 15.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. M. Tsimberidou, P. McLaughlin, A. Younes, M. A. Rodriguez, F. B. Hagemeister, A. Sarris, J. Romaguera, M. Hess, T. L. Smith, Y. Yang, et al.
Fludarabine, mitoxantrone, dexamethasone (FND) compared with an alternating triple therapy (ATT) regimen in patients with stage IV indolent lymphoma
Blood,
December 15, 2002;
100(13):
4351 - 4357.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Viardot, P. Moller, J. Hogel, K. Werner, G. Mechtersheimer, A. D. Ho, G. Ott, T. F.E. Barth, R. Siebert, S. Gesk, et al.
Clinicopathologic Correlations of Genomic Gains and Losses in Follicular Lymphoma
J. Clin. Oncol.,
December 1, 2002;
20(23):
4523 - 4530.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Sanchez-Vega, F. Vega, L. J. Medeiros, M. S. Lee, and R. Luthra
Quantification of bcl-2/JH Fusion Sequences and a Control Gene by Multiplex Real-Time PCR Coupled with Automated Amplicon Sizing by Capillary Electrophoresis
J. Mol. Diagn.,
November 1, 2002;
4(4):
223 - 229.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Hunault-Berger, N. Ifrah, and P. Solal-Celigny
Intensive therapies in follicular non-Hodgkin lymphomas
Blood,
July 30, 2002;
100(4):
1141 - 1152.
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Dolken, F. Schuler, and G. Dolken
Frequency of BCL-2/JH translocation in healthy males exposed to low-level radiation in comparison to age-matched healthy controls
Blood,
July 30, 2002;
100(4):
1513 - 1514.
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. J. Biagi and J. F. Seymour
Insights into the molecular pathogenesis of follicular lymphoma arising from analysis of geographic variation
Blood,
May 29, 2002;
99(12):
4265 - 4275.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Rambaldi, M. Lazzari, C. Manzoni, E. Carlotti, L. Arcaini, M. Baccarani, T. Barbui, C. Bernasconi, G. Dastoli, G. Fuga, et al.
Monitoring of minimal residual disease after CHOP and rituximab in previously untreated patients with follicular lymphoma
Blood,
February 1, 2002;
99(3):
856 - 862.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Zuckerman, T. Zuckerman, D. Sahar, S. Streichman, D. Attias, E. Sabo, D. Yeshurun, and J. M. Rowe
The effect of antiviral therapy on t(14;18) translocation and immunoglobulin gene rearrangement in patients with chronic hepatitis C virus infection
Blood,
March 15, 2001;
97(6):
1555 - 1559.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. B. Kobrin, M. Bendandi, and L. W. Kwak
Novel Secondary Ig VH Gene Rearrangement and In-Frame Ig Heavy Chain Complementarity-Determining Region III Insertion/Deletion Variants in De Novo Follicular Lymphoma
J. Immunol.,
February 15, 2001;
166(4):
2235 - 2243.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. E. Summers, L. K. Goff, A. G. Wilson, R. K. Gupta, T. A. Lister, and J. Fitzgibbon
Frequency of the Bcl-2/IgH Rearrangement in Normal Individuals: Implications for the Monitoring of Disease in Patients With Follicular Lymphoma
J. Clin. Oncol.,
January 15, 2001;
19(2):
420 - 424.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. W. Press, J. F. Eary, T. Gooley, A. K. Gopal, S. Liu, J. G. Rajendran, D. G. Maloney, S. Petersdorf, S. A. Bush, L. D. Durack, et al.
A phase I/II trial of iodine-131-tositumomab (anti-CD20), etoposide, cyclophosphamide, and autologous stem cell transplantation for relapsed B-cell lymphomas
Blood,
November 1, 2000;
96(9):
2934 - 2942.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Cerroni, E. Arzberger, B. Putz, G. Hofler, D. Metze, C. A. Sander, C. Rose, P. Wolf, A. Rutten, J. M. McNiff, et al.
Primary cutaneous follicle center cell lymphoma with follicular growth pattern
Blood,
June 15, 2000;
95(12):
3922 - 3928.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C L Wickham, M Boyce, M V Joyner, P Sarsfield, B S Wilkins, D B Jones, and S Ellard
Amplification of PCR products in excess of 600 base pairs using DNA extracted from decalcified, paraffin wax embedded bone marrow trephine biopsies
Mol. Pathol.,
February 1, 2000;
53(1):
19 - 23.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
T. E. Witzig, C. A. White, G. A. Wiseman, L. I. Gordon, C. Emmanouilides, A. Raubitschek, N. Janakiraman, J. Gutheil, R. J. Schilder, S. Spies, et al.
Phase I/II Trial of IDEC-Y2B8 Radioimmunotherapy for Treatment of Relapsed or Refractory CD20+ B-Cell Non-Hodgkin's Lymphoma
J. Clin. Oncol.,
December 1, 1999;
17(12):
3793 - 3803.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. S. Freedman, D. Neuberg, P. Mauch, R. J. Soiffer, K. C. Anderson, D. C. Fisher, R. Schlossman, E. P. Alyea, T. Takvorian, H. Jallow, et al.
Long-Term Follow-Up of Autologous Bone Marrow Transplantation in Patients With Relapsed Follicular Lymphoma
Blood,
November 15, 1999;
94(10):
3325 - 3333.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Philippe, M.-H. Delfau-Larue, B. Epardeau, B. Autran, J.-P. Clauvel, J.-P. Farcet, and L.-J. Couderc
B-Cell Pulmonary Lymphoma: Gene Rearrangement Analysis of Bronchoalveolar Lymphocytes by Polymerase Chain Reaction
Chest,
May 1, 1999;
115(5):
1242 - 1247.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. A. Hosler, R. O. Bash, X. Bai, V. Jain, and R. H. Scheuermann
Development and Validation of a Quantitative Polymerase Chain Reaction Assay to Evaluate Minimal Residual Disease for T-Cell Acute Lymphoblastic Leukemia and Follicular Lymphoma
Am. J. Pathol.,
April 1, 1999;
154(4):
1023 - 1035.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. S. Czuczman, A. J. Grillo-Lopez, C. A. White, M. Saleh, L. Gordon, A. F. LoBuglio, C. Jonas, D. Klippenstein, B. Dallaire, and C. Varns
Treatment of Patients With Low-Grade B-Cell Lymphoma With the Combination of Chimeric Anti-CD20 Monoclonal Antibody and CHOP Chemotherapy
J. Clin. Oncol.,
January 1, 1999;
17(1):
268 - 268.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. A. Davis, D. G. Maloney, D. K. Czerwinski, T.-M. Liles, and R. Levy
Anti-Idiotype Antibodies Can Induce Long-Term Complete Remissions in Non-Hodgkin's Lymphoma Without Eradicating the Malignant Clone
Blood,
August 15, 1998;
92(4):
1184 - 1190.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Luthra, J. A. McBride, F. Cabanillas, and A. Sarris
Novel 5' Exonuclease-Based Real-Time PCR Assay for the Detection of t(14;18)(q32;q21) in Patients with Follicular Lymphoma
Am. J. Pathol.,
July 1, 1998;
153(1):
63 - 68.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. S. Andersen, J. W. Donovan, J. S. Borus, C. M. Poor, D. Neuberg, J. C. Aster, L. M. Nadler, A. S. Freedman, and J. G. Gribben
Failure of Immunologic Purging in Mantle Cell Lymphoma Assessed by Polymerase Chain Reaction Detection of Minimal Residual Disease
Blood,
November 15, 1997;
90(10):
4212 - 4221.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Lopez, F.M. Lemoine, H. Firat, L. Fouillard, J.P. Laporte, S. Lesage, F. Isnard, J. Stachowiak, F. Ferrer-Le Coeur, P. Morel, et al.
Bone Marrow Versus Peripheral Blood Progenitor Cells CD34 Selection in Patients With Non-Hodgkin's Lymphomas: Different Levels of Tumor Cell Reduction. Implications for Autografting
Blood,
October 1, 1997;
90(7):
2830 - 2838.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Royer, D. Cazals-Hatem, J. Sibilia, F. Agbalika, J.-M. Cayuela, T. Soussi, F. Maloisel, J.-P. Clauvel, J.-C. Brouet, and X. Mariette
Lymphomas in Patients With Sjogren's Syndrome Are Marginal Zone B-Cell Neoplasms, Arise in Diverse Extranodal and Nodal Sites, and Are Not Associated With Viruses
Blood,
July 15, 1997;
90(2):
766 - 775.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. T. Voso, S. Hohaus, M. Moos, and R. Haas
Lack of t(14; 18) Polymerase Chain Reaction-Positive Cells in Highly Purified CD34+ Cells and Their CD19 Subsets in Patients With Follicular Lymphoma
Blood,
May 15, 1997;
89(10):
3763 - 3768.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Corradini, M. Astolfi, C. Cherasco, M. Ladetto, C. Voena, D. Caracciolo, A. Pileri, and C. Tarella
Molecular Monitoring of Minimal Residual Disease in Follicular and Mantle Cell Non-Hodgkin's Lymphomas Treated With High-Dose Chemotherapy and Peripheral Blood Progenitor Cell Autografting
Blood,
January 15, 1997;
89(2):
724 - 491.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D A Molesh and J M Hall
Quantitative analysis of CD34+ stem cells using RT-PCR on whole cells.
Genome Res.,
April 1, 1994;
3(5):
278 - 284.
[Abstract]
[PDF]
|
|
|
|
|
