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Myeloid differentiation of purified CD34+ cells after stimulation with
recombinant human granulocyte-monocyte colony-stimulating factor (CSF),
granulocyte-CSF, monocyte-CSF, and interleukin-3
T Egeland, R Steen, H Quarsten, G Gaudernack, YC Yang and E Thorsby
Institute of Transplantation Immunology, Rikshospitalet University
Hospital, Oslo, Norway.
CD34+ cells isolated from bone marrow or umbilical cord blood from healthy
donors were studied for proliferation and differentiation in liquid
cultures in the presence of recombinant human granulocyte- monocyte
colony-stimulating factor (GM-CSF), granulocyte CSF (G-CSF), monocyte CSF
(M-CSF), and interleukin-3 (IL-3), followed by immunophenotyping for
myeloid and myeloid-associated cell surface markers. IL-3, either alone or
together with GM-CSF, G-CSF, or M-CSF, induced, on average, 50-fold cell
multiplication, GM-CSF five fold to 10-fold, and G-CSF and M-CSF less than
fivefold. Cells from cultures stimulated with GM-CSF, G-CSF, or M-CSF alone
contained cells with a "broad" myeloid profile, "broader" than observed in
cultures with IL-3. However, since IL-3 induced rapid cell multiplication,
high numbers of cells expressing early (CD13, CD33) and late myeloid
markers (CD14, CD15) were recovered. The presence of other CSFs together
with IL-3 did not alter the IL-3-induced effect on the cells. When 5,000
CD34+ cells were cultured with IL-3 alone, the cultures still contained
2,000 to 5,000 CD34+ cells after 14 days of culture, while cells cultured
with GM-CSF, G-CSF, or M-CSF contained less than 1,000 CD34+ cells.
Furthermore, 1,000 to 3,000 cells were positive for the megakaryocytic
lineage marker CD41b after cultures with GM-CSF or IL-3, while cultures
with G-CSF or M-CSF did not contain detectable numbers of CD41b+ cells.
Finally, erythroid cells could also be generated from purified CD34+ cells.
The results show that IL-3 and GM-CSF can induce rapid proliferation of
purified CD34+ cells in vitro with differentiation to multiple myeloid
lineages, while certain subsets maintain expression of CD34.
Volume 78,
Issue 12,
pp. 3192-3199,
12/15/1991
Copyright © 1991 by The American Society of Hematology
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