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CD34 expression by stromal precursors in normal human adult bone marrow
PJ Simmons and B Torok-Storb
Clinical Research Division, Fred Hutchinson Cancer Research Center,
Seattle, WA 98104.
Normal bone marrow cells were isolated by fluorescence-activated cell
sorting (FACS) on the basis of CD34 antigen expression and then assayed in
vitro for colonies of fibroblastic cells (fibroblast colony-forming units
[CFU-F]). Greater than 95% of detectable CFU-F were recovered in the CD34+
population, while their numbers were markedly depleted in the CD34-
population. Additional experiments showed that the majority of CFU-F
exhibited high forward and perpendicular light scatter and low- density
CD34 antigen. Growth of sorted cells in medium optimized for long-term
marrow culture (LTMC) produced a complex mixture of adherent stromal
elements including fibroblasts, adipocytes, smooth muscle cells, and
macrophages. Monoclonal antibody STRO-1, which identifies bone marrow
stromal cells, reacted with approximately 5% of CD34+ cells, which included
all CFU-F and stromal precursors in LTMC. Experiments using soybean
agglutinin (SBA) further showed that these stromal elements were restricted
to a population of bone marrow cells with the phenotype CD34+/SBA+. These
properties of stromal precursors are quite distinct from those of primitive
hematopoietic progenitors, showing that although the precursors of the
hematopoietic and stromal systems share expression of CD34, they are
otherwise phenotypically distinct cell types.
Volume 78,
Issue 11,
pp. 2848-2853,
12/01/1991
Copyright © 1991 by The American Society of Hematology
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