|
|
 Previous Article | Table of Contents | Next Article 
Proliferation of human myeloid leukemia cell line associated with the
tyrosine-phosphorylation and activation of the proto-oncogene c-kit product
A Kuriu, H Ikeda, Y Kanakura, JD Griffin, B Druker, H Yagura, H Kitayama, J Ishikawa, T Nishiura and Y Kanayama
Second Department of Internal Medicine, Osaka University Medical School,
Japan.
We investigated the expression, degree of phosphorylation, and activation
of the proto-oncogene c-kit product before and after stimulation with the
c-kit ligand in a human factor-dependent myeloid leukemia cell line, MO7E.
The culture supernatant of the BALB/3T3 fibroblast cell line, which
contains the ligand for the murine c-kit product, was found to stimulate
proliferation of the MO7E cell line in a dose-dependent manner. The
proliferation was significantly inhibited by a tyrosine kinase inhibitor,
genistein. An immunoblot technique with a monoclonal antibody specific for
phosphotyrosine, showed that there was rapid, dose-dependent
tyrosine-phosphorylation of the c-kit product in response to murine c-kit
ligand. Furthermore, the murine c-kit ligand increased autokinase activity
of the c-kit product in vitro. Similar results were obtained with human
stem cell factor (SCF), a recombinant human ligand for the c-kit product.
These results suggest that the phosphorylation and activation of the c-kit
product are involved in proliferative signals of some human leukemia cells,
as well as of normal hematopoietic cells.
Volume 78,
Issue 11,
pp. 2834-2840,
12/01/1991
Copyright © 1991 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
F. J. Giles, A. T. Stopeck, L. R. Silverman, J. E. Lancet, M. A. Cooper, A. L. Hannah, J. M. Cherrington, A.-M. O'Farrell, H. A. Yuen, S. G. Louie, et al.
SU5416, a small molecule tyrosine kinase receptor inhibitor, has biologic activity in patients with refractory acute myeloid leukemia or myelodysplastic syndromes
Blood,
August 1, 2003;
102(3):
795 - 801.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Hashimoto, I. Matsumura, T. Tsujimura, D.-K. Kim, H. Ogihara, H. Ikeda, S. Ueda, M. Mizuki, H. Sugahara, H. Shibayama, et al.
Necessity of tyrosine 719 and phosphatidylinositol 3'-kinase-mediated signal pathway in constitutive activation and oncogenic potential of c-kit receptor tyrosine kinase with the Asp814Val mutation
Blood,
February 1, 2003;
101(3):
1094 - 1102.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Nollau and B. J. Mayer
Profiling the global tyrosine phosphorylation state by Src homology 2 domain binding
PNAS,
October 31, 2001;
(2001)
241215998.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. D. Smolich, H. A. Yuen, K. A. West, F. J. Giles, M. Albitar, and J. M. Cherrington
The antiangiogenic protein kinase inhibitors SU5416 and SU6668 inhibit the SCF receptor (c-kit) in a human myeloid leukemia cell line and in acute myeloid leukemia blasts
Blood,
March 1, 2001;
97(5):
1413 - 1421.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Beghini, C. B. Ripamonti, P. Peterlongo, G. Roversi, R. Cairoli, E. Morra, and L. Larizza
RNA hyperediting and alternative splicing of hematopoietic cell phosphatase (PTPN6) gene in acute myeloid leukemia
Hum. Mol. Genet.,
September 1, 2000;
9(15):
2297 - 2304.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. C. Heinrich, D. J. Griffith, B. J. Druker, C. L. Wait, K. A. Ott, and A. J. Zigler
Inhibition of c-kit receptor tyrosine kinase activity by STI 571, a selective tyrosine kinase inhibitor
Blood,
August 1, 2000;
96(3):
925 - 932.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D.-K. Kim, E. Morii, H. Ogihara, Y.-M. Lee, T. Jippo, S. Adachi, K. Maeyama, H.-M. Kim, and Y. Kitamura
Different Effect of Various Mutant MITF Encoded by mi, Mior, or Miwh Allele on Phenotype of Murine Mast Cells
Blood,
June 15, 1999;
93(12):
4179 - 4186.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Kozlowski, L. Larose, F. Lee, D. M. Le, R. Rottapel, and K. A. Siminovitch
SHP-1 Binds and Negatively Modulates the c-Kit Receptor by Interaction with Tyrosine 569 in the c-Kit Juxtamembrane Domain
Mol. Cell. Biol.,
April 1, 1998;
18(4):
2089 - 2099.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
V. C. Broudy, N. L. Lin, H.-J. Buhring, N. Komatsu, and T. J. Kavanagh
Analysis of c-kit Receptor Dimerization by Fluorescence Resonance Energy Transfer
Blood,
February 1, 1998;
91(3):
898 - 906.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. C. Vera, A. M. Reyes, J. G. Cárcamo, F. V. Velásquez, C. I. Rivas, R. H. Zhang, P. Strobel, R. Iribarren, H. I. Scher, J. C. Slebe, et al.
Genistein Is a Natural Inhibitor of Hexose and Dehydroascorbic Acid Transport through the Glucose Transporter, GLUT1
J. Biol. Chem.,
April 12, 1996;
271(15):
8719 - 8724.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. H. Jhun, B. Rivnay, D. Price, and H. Avraham
The MATK Tyrosine Kinase Interacts in a Specific and SH2-dependent Manner with c-Kit
J. Biol. Chem.,
April 21, 1995;
270(16):
9661 - 9666.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Li, J. J. Macpherson, S. Adelstein, C. L. Bunn, K. Atkinson, K. Phadke, and S. A. Krilis
Conditioned Media from a Cell Strain Derived from a Patient with Mastocytosis Induces Preferential Development of Cells That Possess High Affinity IgE Receptors and the Granule Protease Phenotype of Mature Cutaneous Mast Cells
J. Biol. Chem.,
February 3, 1995;
270(5):
2258 - 2263.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Nollau and B. J. Mayer
Profiling the global tyrosine phosphorylation state by Src homology 2 domain binding
PNAS,
November 20, 2001;
98(24):
13531 - 13536.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
