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T-lymphocytic leukemia expresses complex, branched O-linked
oligosaccharides on a major sialoglycoprotein, leukosialin
O Saitoh, F Piller, RI Fox and M Fukuda
La Jolla Cancer Research Foundation, Cancer Research Center, CA 92037.
Leukocytes express a major sialoglycoprotein, leukosialin, of which the
apparent molecular weight (mol wt) can be variable according to the
differences in O-glycans attached to this molecule. In the present study,
we analyzed the structures of O-glycans attached to leukosialin present in
various T-lymphocytic leukemia cells. T-lymphoid cells from patients with
acute T-lymphocytic leukemia express a large amount of the branched
hexasaccharides, NeuNAc alpha 2----3Gal beta 1----3(NeuNAc alpha 2----3Gal
beta 1----4GlcNAc beta 1----6)GalNAc, which are also expressed in activated
normal T lymphocytes, but that are almost absent in resting normal T
lymphocytes. T-lymphoid cells from patients with chronic T-lymphocytic
leukemia, on the other hand, mainly express the tetrasaccharides NeuNAc
alpha 2----3Gal beta 1----3(NeuNAc alpha 2---- 6)GalNAc on leukosialin, but
they also express a small significant amount of the hexasaccharides. The
same hexasaccharides can be detected in thymocytes. The increased amount of
the hexasaccharides in acute leukemia is associated with increased activity
of beta 1----6 GlcNAc- transferase, a key enzyme in forming the
hexasaccharides. Immunoblot analysis of cell lysates showed that monoclonal
antibody (MoAb) T-305 reacts preferentially with leukosialin of high mol wt
containing the hexasaccharides. These findings indicate that T-lymphocytic
leukemia cells reexpress the oligosaccharides present in immature cells.
Volume 77,
Issue 7,
pp. 1491-1499,
04/01/1991
Copyright © 1991 by The American Society of Hematology
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|
|
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|
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|
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|
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