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In vivo effect of human granulocyte-macrophage colony-stimulating factor on
megakaryocytopoiesis
M Aglietta, C Monzeglio, F Sanavio, F Apra, S Morelli, A Stacchini, W Piacibello, F Bussolino, G Bagnara and G Zauli
Dipartimento di Scienze Biomediche ed Oncologia Umana, Universita di
Torino, Italy.
The effect of granulocyte-macrophage colony-stimulating factor (GM-CSF) on
megakaryocytopoiesis and platelet production was investigated in patients
with normal hematopoiesis. Three findings indicated that GM- CSF plays a
role in megakaryocytopoiesis. During treatment with GM-CSF (recombinant
mammalian, glycosylated; Sandoz/Schering-Plough, 5.5 micrograms
protein/kg/d, subcutaneously for 3 days) the percentage of megakaryocyte
progenitors (megakaryocyte colony forming unit [CFU-Mk]) in S phase
(evaluated by the suicide technique with high 3H-Tdr doses) increased from
31% +/- 16% to 88% +/- 11%; and the maturation profile of megakaryocytes
was modified, with a relative increase in more immature stage I-III forms.
Moreover, by autoradiography (after incubation of marrow cells with
125I-labeled GM-CSF) specific GM-CSF receptors were detectable on
megakaryocytes. Nevertheless, the proliferative stimulus induced on the
progenitors was not accompanied by enhanced platelet production (by
contrast with the marked granulomonocytosis). It may be suggested that
other cytokines are involved in the regulation of the intermediate and
terminal stages of megakaryocytopoiesis in vivo and that their intervention
is an essential prerequisite to turn the GM-CSF-induced proliferative
stimulus into enhanced platelet production.
Volume 77,
Issue 6,
pp. 1191-1194,
03/15/1991
Copyright © 1991 by The American Society of Hematology
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