|
|
 Previous Article | Table of Contents | Next Article 
B-lineage colonies from normal, human bone marrow are initiated by B cells
and their progenitors
K McGinnes, M Letarte and CJ Paige
Division of Cell and Molecular Biology, Ontario Cancer Institute, Toronto,
Canada.
We have recently described a reproducible method whereby colonies
containing cells that secrete immunoglobulin (Ig) can be grown from normal,
human, adult bone marrow samples. The present report characterizes the
cells that initiate these colonies. It is shown that all clonogenic cells
express the CD19 surface antigen, as removal of these cells before plating
in the B-cell colony assay abolished the subsequent growth of
plaque-forming, B-lineage colonies. Cells from both the CD10+ and CD20+
B-lineage subpopulations initiated the growth of B-cell colonies, as
removal of either subset resulted in a 50% reduction in the number of
resulting B-cell colonies. The removal of activated B cells (CD23+), plasma
cells (PCA-1+), or myeloid cells (CD13+) did not lead to a significant
depletion in B-cell colony formation. Pre-B cells that were not yet
committed to Ig light chain expression were also able to differentiate and
proliferate into Ig- secreting colonies under the culture conditions used.
Colonies initiated by these light chain uncommitted cells were
distinguished using a replicate protein immunoblotting technique, which
detects the simultaneous secretion of Ig kappa and Ig lambda from single
colonies. These experiments provide evidence that the CD10 antigen is
expressed on B-lineage cells before Ig light chain commitment, whereas CD20
is not. In conclusion, this B-cell colony assay provides a system for
studying the differentiation of bone marrow-derived B cells and their
precursors into Ig-secreting cells.
Volume 77,
Issue 5,
pp. 961-970,
03/01/1991
Copyright © 1991 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
F. Heller, M. T. Lindenmeyer, C. D. Cohen, U. Brandt, D. Draganovici, M. Fischereder, M. Kretzler, H.-J. Anders, T. Sitter, I. Mosberger, et al.
The Contribution of B Cells to Renal Interstitial Inflammation
Am. J. Pathol.,
February 1, 2007;
170(2):
457 - 468.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A.-C. Fluckiger, E. Sanz, M. Garcia-Lloret, T. Su, Q.-L. Hao, R. Kato, S. Quan, A. de la Hera, G. M. Crooks, O. N. Witte, et al.
In Vitro Reconstitution of Human B-Cell Ontogeny: From CD34+ Multipotent Progenitors to Ig-Secreting Cells
Blood,
December 15, 1998;
92(12):
4509 - 4520.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. C. Berardi, E. Meffre, F. Pflumio, A. Katz, W. Vainchenker, C. Schiff, and L. Coulombel
Individual CD34+CD38lowCD19-CD10- Progenitor Cells From Human Cord Blood Generate B Lymphocytes and Granulocytes
Blood,
May 15, 1997;
89(10):
3554 - 3564.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
