The beta globin 3' enhancer element confers regulated expression on the
human gamma globin gene in the human embryonic-fetal erythroleukemia cell
line K562
M Donovan-Peluso, S Acuto, D O'Neill, A Hom, A Maggio and A Bank
Department of Medicine, College of Physicians and Surgeons, Columbia
University, New York, NY 10032.
We have constructed fusion genes comprised of gamma and beta globin
elements and globin sequences linked to neomycin resistance (neoR) genes to
define the cis acting sequences responsible for developmental
stage-specific expression and induction of fetal globin genes in
embryonic-fetal erythroleukemia K562 cells. The results indicate that the
gamma promoter is required for proper initiation of transcription. However,
the accumulation of gamma globin transcripts in response to hemin induction
requires the additional presence of either gamma intervening sequence 2 or
the 3' enhancer element of the beta globin gene. Thus, the gamma promoter
may provide the elements for developmental stage-specific gene expression
during fetal life. By contrast, the beta 3' enhancer is erythroid-specific
but not developmental stage- or gene-specific.
Volume 77,
Issue 4,
pp. 855-860,
02/15/1991
Copyright © 1991 by The American Society of Hematology
