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Transmembrane mobility of phospholipids in sickle erythrocytes: effect of
deoxygenation on diffusion and asymmetry
N Blumenfeld, A Zachowski, F Galacteros, Y Beuzard and PF Devaux
Laboratoire de Biochimie, Hopital Henri Mondor, Creteil, France.
We studied the effect of sickling on the transmembrane reorientation and
distribution of phospholipids in the red blood cells of patients homozygous
for sickle cell anemia (SS). To this purpose, we followed the
redistribution kinetics of trace amounts of spin-labeled analogues of
natural phospholipids first introduced in the membrane outer leaflet of
normal or sickle erythrocytes exposed to air or nitrogen. Deoxygenation had
no effect on the lipid redistribution kinetics in normal (AA) cell
membranes. At atmospheric pO2, unfractionated SS cells were not different
from normal cells. However, on deoxygenation inducing sickling,
phosphatidylcholine passive diffusion was accelerated and the rate of the
adenosine triphosphate-dependent transport of aminophospholipids was
reduced, especially for phosphatidylserine. The stationary distribution of
the aminophospholipids between the two leaflets was slightly less
asymmetric, a phenomenon more pronounced with phosphatidylethanolamine.
These changes were rapidly reversible on reoxygenation. When SS cells were
separated by density, both dense and light cells exhibited the properties
cited above. However, dense cells exposed to air possessed a lower
aminophospholipid transport rate. These data favor the relationship between
aminophospholipid translocase activity and phospholipid transmembrane
asymmetry. Sickle cell disease is the first case of aminophospholipid
translocase pathology.
Volume 77,
Issue 4,
pp. 849-854,
02/15/1991
Copyright © 1991 by The American Society of Hematology
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