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Analysis of immunoglobulin and T-cell receptor gene rearrangements in human fetal bone marrow B lineage cells

TW LeBien, RL Elstrom, M Moseley, JH Kersey and F Griesinger

Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis 55455.

Fetal bone marrow B lineage cells representing multiple stages of B cell development were isolated by two-color cell sorting and analyzed for immunoglobulin H and T-cell receptor (TCR) gamma and delta gene rearrangements. Analysis of CD10+/surface mu- cells using a JH probe revealed a high frequency of rearrangements; some of these rearrangements used the 3' D region gene DQ52. Analysis of CD10+/surface mu- cells revealed no detectable TCR-gamma or -delta rearrangements, nor were TCR-delta rearrangements detected in CD10+/surface mu+ cells, despite the limited repertoire of these genes. These observations are surprising given the high frequency of TCR delta/gamma rearrangements in B cell precursor acute lymphoblastic leukemia, and identify a potential difference in patterns of gene rearrangement that distinguish normal and leukemic B cell precursors.

Volume 76, Issue 6, pp. 1196-1200, 09/15/1990
Copyright © 1990 by The American Society of Hematology


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  Copyright © 1990 by American Society of Hematology         Online ISSN: 1528-0020