Interferon affects nuclear proteins in cells of clinically sensitive
chronic myelogenous leukemia patients
OM Howard, M Talpaz, H Kantarjian, D Seong, A Wedrychowski, N Paslidis, J Hester, A Cork, J Turpin and G Lopez-Berestein
Department of Hematology, University of Texas M.D. Anderson Cancer Center,
Houston, TX.
Cytoplasmic protein extracts from chronic myelogenous leukemia (CML) cells
contained an activity that altered the electrophoretic mobility of
complexes formed between nuclear proteins and the transcriptional enhancers
of interferon (IFN)-inducible genes. Exposure of CML cells to IFN-alpha
diminished the effect of the CML cytoplasmic proteins on these nuclear
protein-DNA complexes. The presence of clinical responsiveness to IFN-alpha
correlated with the sensitivity to the IFN- induced change in the
electrophoretic mobility of nuclear protein-DNA complexes. These data
suggest that the action of IFN-alpha in CML may be linked to a pathway that
can result in posttranslational modification of nuclear proteins.
Volume 76,
Issue 6,
pp. 1117-1130,
09/15/1990
Copyright © 1990 by The American Society of Hematology
