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Expression of the CD11/CD18, leukocyte adhesion molecule 1, and CD44
adhesion molecules during normal myeloid and erythroid differentiation in
humans
GS Kansas, MJ Muirhead and MO Dailey
Department of Pathology, University of Iowa College of Medicine, Iowa City.
We have used three-color flow cytometry to investigate the pattern of
expression of the CD11/CD18, CD44, and leukocyte adhesion molecule 1
(LAM-1) adhesion molecules during myeloid and erythroid differentiation in
humans. The earliest myeloid cells, identified as CD33loCD15-, were
exclusively CD44hi but contained both leukocyte function-associated antigen
1 (LFA-1hi) and LFA-1lo cells, as well as LAM-1+ and LAM-1- cells. This
CD33loCD15- myeloid subpopulation expressed only low levels of CD11c and
failed to express CD11b, CD14, or any lymphoid (CD3, CD16, CD19) antigens
or glycophorin. Commitment to monocyte differentiation, suggested by the
presence of an LFA-1hi CD11c+ subset within the CD33loCD15- subpopulation,
was clearly signaled by upregulation of CD33; these monocyte-lineage
committed cells were exclusively CD33hi, CD44hi, CD11ahi, CD11c+, and
exhibited a broad range of intensity of CD15 expression. Later stages of
monopoiesis were identified by acquisition of CD11b, and subsequently of
CD14. Myeloid cells committed to granulopoiesis remained LFA-1lo, and
underwent a sharp upregulation of CD15 along with downregulation of both
CD33 and CD44. Successive stages of granulocyte development were marked by
expression of CD11b and, subsequently, of CD16. The earliest cells capable
of erythroid differentiation were CD44hi, LFA-1lo, and LAM-1+. Both LFA-1
and LAM-1 were lost before the onset of glycophorin (glyco) expression,
whereas CD44 expression remained high on glyco+ cells, which also expressed
CD45. CD44 expression was intermediate on glyco+ CD71+ cells, and low on
glyco+ CD45- CD71- cells, similar to normal, circulating erythrocytes. Our
results allow us to phenotypically define discrete stages in the normal
development of monocytes, neutrophils, and erythrocytes. The expression of
LFA-1, LAM-1, and high levels of CD44 on the most primitive hematopoietic
cells detectable by flow cytometry suggests that at least some of these
molecules are critically involved in leukocyte adhesion during development.
Volume 76,
Issue 12,
pp. 2483-2492,
12/15/1990
Copyright © 1990 by The American Society of Hematology
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