In vivo hematologic effects of recombinant human macrophage colony-
stimulating factor
TR Ulich, J del Castillo, LR Watson, SM Yin and MB Garnick
Department of Pathology, University of California, Irvine 92717.
Macrophage colony-stimulating factor (recombinant human M-CSF) given as a
single intravenous injection to Lewis rats induces a dose-dependent
peripheral monocytosis, neutrophilia, and lymphopenia. The monocytosis
peaks at 28 to 32 hours with a seven- to eightfold increase in the number
of circulating monocytes and promonocytes. The peripheral monocytosis is
accompanied by a slight increase in marrow blasts, promonocytes, and
monocytes. A monocytopenia reaching a nadir at 15 minutes precedes the
monocytosis, suggesting that M-CSF activates circulating monocytes and
causes intravascular margination. The M-CSF- induced neutrophilia and
lymphopenia are relatively mild in magnitude, are observed between 2 and 16
hours after injection, and are no longer evident at later time-points. The
monocytosis was at least partially inhibited by dexamethasone.
M-CSF-induced monocytosis most likely reflects a direct effect of M-CSF on
marrow monocyte precursor proliferation, maturation, and release, whereas
the neutrophilia and lymphopenia may reflect indirect effects mediated by
the known ability of M-CSF to cause the release of other cytokines.
Volume 75,
Issue 4,
pp. 846-850,
02/15/1990
Copyright © 1990 by The American Society of Hematology
