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Identification of the platelet-specific alloantigen, Naka, on platelet
membrane glycoprotein IV
Y Tomiyama, H Take, H Ikeda, T Mitani, T Furubayashi, H Mizutani, N Yamamoto, NN Tandon, S Sekiguchi and GA Jamieson
Second Department of Internal Medicine, Osaka University Medical School,
Japan.
We describe the membrane localization of a new platelet-specific
alloantigen, designated Naka, that is involved in refractoriness to HLA-
matched platelet transfusions. By indirect immunoprecipitation, anti- Naka
antibody precipitated a single, radiolabeled platelet membrane protein with
a molecular weight (mol wt) of 91 Kd from Naka-positive platelets. When
radiolabeled Naka-negative platelets were used as a source of target
antigens, no radiolabeled proteins were precipitated. The analyses using
nonreduced-reduced two-dimensional sodium dodecyl sulfate-polyacrylamide
gel electrophoresis (SDS-PAGE) and using rabbit antiglycoprotein (GP)IV
demonstrated that this protein corresponds to GPIV (alternatively GPIIIb).
Furthermore, in dot immunobinding, anti- Naka antibody bound to purified
GPIV. Our results provide definitive evidence that the Naka alloantigen is
carried on GPIV. These results also demonstrate that, on occasion,
antibodies against GPIV may play an important role in refractoriness to
platelet transfusions.
Volume 75,
Issue 3,
pp. 684-687,
02/01/1990
Copyright © 1990 by The American Society of Hematology
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