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Bone marrow stromal cell regulation of B lymphopoiesis: interleukin-1
(IL-1) and IL-4 regulate stromal cell support of pre-B cell production in
vitro
LG Billips, D Petitte and KS Landreth
Department of Microbiology and Immunology, West Verginia University Health
Sciences Center, Morgantown 26506.
Bone marrow stromal cells appear to be key regulatory elements in
hematopoiesis and lymphopoiesis. These stromal cells respond to cytokine
exposure and alter their pattern of hematopoietic growth factor production,
suggesting a degree of functional plasticity. We examined the effect of two
cytokines, interleukin-1 (IL-1) and IL-4, on stromal cell regulation of
pre-B cell generation using the bone marrow stromal cell line, S17. Neither
lymphokine potentiated pre-B cell generation in the absence of stromal
cells. However, addition of either 10 U/mL rIL-1 alpha or 50 U/mL rIL-4 to
cultures of bone marrow cells containing S17 cells dramatically suppressed
subsequent pre-B cell formation. Preculture of S17 stromal cells with
either rIL-1 or rIL-4 completely abrogated their ability to support pre-B
cell generation in subsequent coculture with freshly explanted bone marrow
cells. Conditioned medium from IL-1- or IL-4-treated S17 cells also
suppressed pre-B-cell generation in culture. Although it is not yet known
which induced stromal cell factors are responsible for failure of
pre-B-cell generation in treated cultures, these data do clearly
demonstrate that local levels of IL-1 and IL-4 in the hematopoietic
microenvironment may play a significant role in regulation of bone marrow
stromal cell function. These data also demonstrate that fibroblastic
stromal cells are primary target cells that respond to cytokine
concentration and affect lymphopoietic cell development.
Volume 75,
Issue 3,
pp. 611-619,
02/01/1990
Copyright © 1990 by The American Society of Hematology
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