|
|
 Previous Article | Table of Contents | Next Article 
Transforming growth factor beta directly regulates primitive murine
hematopoietic cell proliferation
JR Keller, IK Mcniece, KT Sill, LR Ellingsworth, PJ Quesenberry, GK Sing and FW Ruscetti
Laboratory of Molecular Immunoregulation, Program Resources, Inc, National
Cancer Institute, Frederick, MD 21701.
We previously reported that transforming growth factor beta (TGF-beta)
selectively inhibits colony-stimulating factor-driven hematopoietic
progenitor cell growth. We report here that TGF-beta 1 can act directly on
hematopoietic progenitors to inhibit the growth of the most primitive
progenitors measurable in vitro. Highly enriched populations of
hematopoietic progenitor cells were obtained by isolating lineage negative
(Lin-), Thy-1-positive (Thy-1+) fresh bone marrow cells, or by isolating
cells from interleukin-3 (IL-3) supplemented bone marrow cultures
expressing Thy-1 antigen with the fluorescent activated cell sorter.
TGF-beta 1 inhibited IL-3-induced Thy-1 expression on Thy-1- negative
(Thy-1-) bone marrow cells in a dose-dependent manner with an ED50 of 5 to
10 pmol/L. In addition, TGF-beta 1 inhibited the formation of multipotent
and mixed colonies by isolated Thy-1+ cells, while single lineage
granulocyte and macrophage colonies were not affected. The growth of Thy-1+
Lin- cells incubated as single cells in Terasaki plates in medium
supplemented with IL-3 were inhibited by TGF-beta, demonstrating a direct
inhibitory effect. Hematopoietic stem cells, which have a high
proliferative potential (HPP) when responding to combinations of growth
factors in vitro, have been detected in the bone marrow of normal mice and
mice surviving a single injection of 5- fluorouracil. TGF-beta 1 inhibited
the growth of all subpopulations of HPP colony forming cells (CFC) in a
dose-dependent manner with an ED50 of 5 to 10 pmol/L. Thus, TGF-beta
directly inhibits the growth of the most immature hematopoietic cells
measurable in vitro.
Volume 75,
Issue 3,
pp. 596-602,
02/01/1990
Copyright © 1990 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
U. Blank, G. Karlsson, J. L. Moody, T. Utsugisawa, M. Magnusson, S. Singbrant, J. Larsson, and S. Karlsson
Smad7 promotes self-renewal of hematopoietic stem cells
Blood,
December 15, 2006;
108(13):
4246 - 4254.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Utsugisawa, J. L. Moody, M. Aspling, E. Nilsson, L. Carlsson, and S. Karlsson
A Road Map Toward Defining the Role of Smad Signaling in Hematopoietic Stem Cells
Stem Cells,
April 1, 2006;
24(4):
1128 - 1136.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Yang, I. Dybedal, D. Bryder, L. Nilsson, E. Sitnicka, Y. Sasaki, and S. E. W. Jacobsen
IFN-{gamma} Negatively Modulates Self-Renewal of Repopulating Human Hemopoietic Stem Cells
J. Immunol.,
January 15, 2005;
174(2):
752 - 757.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Heath, H. C. Suh, M. Holman, K. Renn, J. M. Gooya, S. Parkin, K. D. Klarmann, M. Ortiz, P. Johnson, and J. Keller
C/EBP{alpha} deficiency results in hyperproliferation of hematopoietic progenitor cells and disrupts macrophage development in vitro and in vivo
Blood,
September 15, 2004;
104(6):
1639 - 1647.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Klarmann, M. Ortiz, M. Davies, and J. R. Keller
Identification of in vitro growth conditions for c-Kit-negative hematopoietic stem cells
Blood,
November 1, 2003;
102(9):
3120 - 3128.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Liu, Y. Sun, F. Jiang, S. Zhang, Y. Wu, Y. Lan, X. Yang, and N. Mao
Disruption of Smad5 gene leads to enhanced proliferation of high-proliferative potential precursors during embryonic hematopoiesis
Blood,
January 1, 2003;
101(1):
124 - 133.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Chagraoui, E. Komura, M. Tulliez, S. Giraudier, W. Vainchenker, and F. Wendling
Prominent role of TGF-beta 1 in thrombopoietin-induced myelofibrosis in mice
Blood,
November 15, 2002;
100(10):
3495 - 3503.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. H. Shah, W. B. Tabayoyong, S. Y. Kimm, S.-J. Kim, L. van Parijs, and C. Lee
Reconstitution of Lethally Irradiated Adult Mice with Dominant Negative TGF-{beta} Type II Receptor-Transduced Bone Marrow Leads to Myeloid Expansion and Inflammatory Disease
J. Immunol.,
October 1, 2002;
169(7):
3485 - 3491.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Kouro, K. L. Medina, K. Oritani, and P. W. Kincade
Characteristics of early murine B-lymphocyte precursors and their direct sensitivity to negative regulators
Blood,
May 1, 2001;
97(9):
2708 - 2715.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. O. Fortunel, A. Hatzfeld, and J. A. Hatzfeld
Transforming growth factor-beta : pleiotropic role in the regulation of hematopoiesis
Blood,
September 15, 2000;
96(6):
2022 - 2036.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Fortunel, J. Hatzfeld, S. Kisselev, M.-N. Monier, K. Ducos, A. Cardoso, P. Batard, and A. Hatzfeld
Release from Quiescence of Primitive Human Hematopoietic Stem/Progenitor Cells by Blocking Their Cell-Surface TGF-{beta} Type II Receptor in a Short-Term In Vitro Assay
Stem Cells,
March 1, 2000;
18(2):
102 - 111.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
P Batard, M. Monier, N Fortunel, K Ducos, P Sansilvestri-Morel, T Phan, A Hatzfeld, and J. Hatzfeld
TGF-(beta)1 maintains hematopoietic immaturity by a reversible negative control of cell cycle and induces CD34 antigen up-modulation
J. Cell Sci.,
January 2, 2000;
113(3):
383 - 390.
[Abstract]
[PDF]
|
|
|
|
|
|
|
J. B. Paukovits, R. Rutter, E. Ganglberger, H. I. Karlic, B. Marian, and W. R. Paukovits
The Hemoregulatory Peptide pEEDCK May Inhibit Stem Cell Proliferation via Hydropathic Binding to Antisense Sequence Motifs in Interleukin-11 and Other Growth Factors
Mol. Pharmacol.,
October 1, 1999;
56(4):
665 - 674.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
G. Barosi
Myelofibrosis With Myeloid Metaplasia: Diagnostic Definition and Prognostic Classification for Clinical Studies and Treatment Guidelines
J. Clin. Oncol.,
September 1, 1999;
17(9):
2954 - 2954.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. E. Purton, I. D. Bernstein, and S. J. Collins
All-Trans Retinoic Acid Delays the Differentiation of Primitive Hematopoietic Precursors (lin-c-kit+Sca-1+) While Enhancing the Terminal Maturation of Committed Granulocyte/Monocyte Progenitors
Blood,
July 15, 1999;
94(2):
483 - 495.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V.P. Kale and L.S. Limaye
Stimulation of Adult Human Bone Marrow by Factors Secreted by Fetal Liver Hematopoietic Cells: In Vitro Evaluation Using Semisolid Clonal Assay System
Stem Cells,
March 1, 1999;
17(2):
107 - 116.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. M. Robledo, F. Sanz-Rodriguez, A. Hidalgo, and J. Teixido
Differential Use of Very Late Antigen-4 and -5 Integrins by Hematopoietic Precursors and Myeloma Cells to Adhere to Transforming Growth Factor-beta 1-treated Bone Marrow Stroma
J. Biol. Chem.,
May 15, 1998;
273(20):
12056 - 12060.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
