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Hematopoietic progenitor cell expression of the H-CAM (CD44) homing-
associated adhesion molecule
DM Lewinsohn, A Nagler, N Ginzton, P Greenberg and EC Butcher
Department of Pathology, Stanford University School of Medicine, CA
94305-5324.
We explored the expression of a lymphocyte homing-associated cell adhesion
molecule (H-CAM, CD44) on hematopoietic progenitors. We demonstrate that
immature myeloid and erythroid leukemic cell lines stain intensely with
monoclonal antibodies Hermes-1 and Hermes-3, which define distinct epitopes
on lymphocyte surface H-CAM, a glycoprotein involved in lymphocyte
interactions with endothelial cells. Using fluorescence-activated cell
sorting (FACS), human marrow cells were fractionated into Hermeshi,
Hermesmed, and Hermeslo populations according to the expression of both the
Hermes-1 and Hermes-3 epitopes. Granulocyte-macrophage colony-forming unit
and erythroid burst-forming unit precursors were found predominantly in the
brightly positive fractions. Two-color FACS analysis confirmed that the
My10 (CD34) positive populations of cells in bone marrow, which contain
most of the progenitor cell activity, are brightly positive for Hermes-1.
Finally, we demonstrate that among bone marrow cells, the highest levels of
H- CAM are expressed on myeloid and erythroid progenitors as well as mature
granulocytes and lymphocytes. Thus we provide evidence that molecules
related or identical to the H-CAM homing receptor are expressed on marrow
progenitor cells. H-CAM may contribute to progenitor cell interactions with
marrow endothelial and stromal cell elements important to the maintenance
and regulation of hematopoiesis.
Volume 75,
Issue 3,
pp. 589-595,
02/01/1990
Copyright © 1990 by The American Society of Hematology
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