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Blood-borne collagenous debris complexes with plasma fibronectin after
thermal injury
P La Celle, FA Blumenstock, C McKinley, TM Saba, PA Vincent and V Gray
Department of Physiology, Albany Medical College, NY 12208.
Plasma fibronectin augments the clearance of blood-borne foreign and effete
complexes by mononuclear phagocytes. The release of a "gelatin- like"
ligand into plasma after thermal injury has been reported. We quantified
the release of this collagenous debris from thermally injured skin, and its
potential interaction with soluble fibronectin in plasma using anesthetized
rats. Collagen-like material debris in the plasma was detected by assay of
hydroxyproline. Fibronectin was measured by a double antibody enzyme-linked
immunosorbent assay (ELISA) technique. Over a 24-hour postburn interval,
plasma hydroxyproline increased from 6.7 +/- 0.6 micrograms/mL to a maximum
of 19.0 +/- 3.3 micrograms/mL at 60 minutes postburn, and normalized by 6
hours. A direct correlation existed between the magnitude of burn injury
and the increase in plasma hydroxyproline. In parallel, plasma fibronectin
declined over a 15-minute to 2-hour period postburn, and normalized by 3 to
4 hours with rebound hyperfibronectinemia observed at 24 hours. The
elevation in total plasma hydroxyproline was not due to an increase in
plasma Clq (zero time, 26.2 +/- 1.4 micrograms/mL; 60 minutes, 23.9 +/- 1.1
micrograms/mL). Tracer studies with 125I-fibronectin showed that the acute
decline of plasma fibronectin was due to its uptake by the liver and
binding to sites of tissue injury. Total hydroxyproline in extracts of burn
skin, used as an index of soluble collagenous material, rose from 15 +/-
3.3 micrograms/g skin at zero time to 129.3 +/- 43.7 micrograms/g skin by 5
minutes postburn, with a decline to 38 +/- 22 micrograms/g skin by 24
hours. The formation of circulating fibronectin-gelatin complexes in vivo
was documented by cross- immunoelectrophoresis coupled with autoradiography
using 125I-gelatin as a model ligand. Thus, collagenous tissue debris from
burned skin may enter the plasma after thermal injury and directly
complexes with soluble fibronectin before hepatic phagocytic clearance.
Volume 75,
Issue 2,
pp. 470-478,
01/15/1990
Copyright © 1990 by The American Society of Hematology
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