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Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  High human T-cell lymphotropic virus type I proviral DNA load with polyclonal integration in peripheral blood mononuclear cells of French West Indian, Guianese, and African patients with tropical spastic paraparesis A Gessain, F Saal, O Gout, MT Daniel, G Flandrin, G de The, J Peries and F Sigaux Laboratoire d'Hematologie Moleculaire, Hopital Saint-Louis, France. Human T-cell lymphotropic virus type I (HTLV-I) proviral integration status was examined by Southern blot analysis in peripheral blood mononuclear cell (PBMC) DNA from patients presenting a tropical spastic paraparesis (TSP) and serological evidence of HTLV-I infection. Surface phenotype and morphological aspects of PBMC were also studied. A polyclonal HTLV-I proviral integration was found in the PBMC of the 10 patients studied irrespective of their geographical origin (French West Indies, French Guiana, and Africa), the duration of their clinical illness, or the HTLV-I antibody titer. Furthermore, by dilution experiments and hypothesizing that only one copy of HTLV-I proviral DNA is present in one cell, we estimated that this HTLV-I integration is present in 3% to 15% of their PBMC. All 10 TSP/HTLV-I patients studied had an average of 10% of their lymphocytes abnormal, presenting either a misshapen nucleus or an adult T-cell leukemia/lymphoma (ATL)-like feature. Moreover, an elevated CD4/CD8 ratio associated with the presence of activated T cells with a high level of DR expression was observed in most patients. The significant frequency of viral-positive PBMC and the important load of HTLV-I proviral DNA that we observed in TSP/HTLV-I patients might play an important role in the pathogenesis of this recently identified clinico-virological entity. Volume 75, Issue 2, pp. 428-433, 01/15/1990 Copyright © 1990 by The American Society of Hematology CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: N. Dumais, M.-E. Pare, S. Mercier, S. Bounou, S. J. Marriot, B. Barbeau, and M. J. Tremblay T-Cell Receptor/CD28 Engagement When Combined with Prostaglandin E2 Treatment Leads to Potent Activation of Human T-Cell Leukemia Virus Type 1 J. Virol., October 15, 2003; 77(20): 11170 - 11179. [Abstract] [Full Text] [PDF] T. Koiwa, A. Hamano-Usami, T. Ishida, A. Okayama, K. Yamaguchi, S. Kamihira, and T. 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	Copyright © 1990 by American Society of Hematology         Online ISSN: 1528-0020