Proliferative properties of unfractionated, purified, and single cell human
progenitor populations stimulated by recombinant human interleukin-3
G Kannourakis and GR Johnson
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne
Hospital, Victoria, Australia.
In this report, the biological properties of human recombinant
interleukin-3 (rhIL-3) were studied. We investigated the range of
unfractionated, purified and single cell human progenitors responsive to
IL-3; compared the colony types observed with those obtained in the
presence of recombinant human granulocyte-macrophage colony-stimulating
factor (GM-CSF) or granulocyte-CSF (G-CSF). The results show that IL-3
directly stimulates the formation of colonies derived from eosinophil and,
to a lesser degree, granulocyte and macrophage progenitors. In combination
with erythropoietin, it supports the development of erythroid and
mixed-erythroid colonies. Furthermore, the data show that IL-3 is a more
potent stimulus for both erythroid and eosinophil progenitors than GM-CSF.
Interleukin-3 stimulates the formation of both compact and dispersed
colonies derived from eosinophil progenitors, whereas GM-CSF stimulates the
formation of only the compact type. We conclude that some of the
proliferative effects of IL-3 observed on unfractionated and semipurified
bone marrow populations are indirect and most likely involve accessory cell
interactions.
Volume 75,
Issue 2,
pp. 370-377,
01/15/1990
Copyright © 1990 by The American Society of Hematology
