|
|
 Previous Article | Table of Contents | Next Article 
Polarized fibronectin secretion and localized matrix assembly sites
correlate with subendothelial matrix formation
AP Kowalczyk, RH Tulloh and PJ McKeown-Longo
Department of Physiology and Cell Biology, Albany Medical College, NY
12208.
Endothelial cells in vivo form the interface between the vascular and
interstitial compartments and are strategically located to mediate vascular
permeability and hemostasis. One mechanism endothelial cells use to
maintain a nonthrombogenic surface is to polarize basement membrane
constituents to the basolateral cell surface. In the present study, we
began characterization of the mechanisms used by endothelial cells in the
assembly of a subcellular fibronectin matrix. Immunofluorescence microscopy
was used to localize extracellular matrix fibronectin in endothelial cell
cultures. In contrast to preconfluent and newly confluent cultures,
post-confluent cultures assembled a fibronectin matrix that was restricted
to the basolateral cell surface. To determine if endothelial cells polarize
fibronectin secretion, Millicell culture inserts were used to distinguish
proteins secreted from apical and basal surfaces. Preconfluent and newly
confluent cultures secreted fibronectin equally into apical and basal
media. In contrast, post-confluent endothelial cells secreted fibronectin
preferentially into the basal chamber. The degree to which fibronectin
secretion was polarized varied with time in culture and with the ability of
the monolayers to act as a barrier to the movement of 125I- fibronectin
from the apical to basal chamber. In addition, high affinity binding sites
for exogenous 125I-fibronectin were found to be present on the basolateral,
but not apical, surface of post-confluent endothelial monolayers. These
results indicate that subendothelial matrix assembly correlates with
polarized fibronectin secretion, culture confluence, and expression of high
affinity binding sites for fibronectin on the basolateral cell surface.
Volume 75,
Issue 12,
pp. 2335-2342,
06/15/1990
Copyright © 1990 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
F. A. Moretti, A. K. Chauhan, A. Iaconcig, F. Porro, F. E. Baralle, and A. F. Muro
A Major Fraction of Fibronectin Present in the Extracellular Matrix of Tissues Is Plasma-derived
J. Biol. Chem.,
September 21, 2007;
282(38):
28057 - 28062.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Erdreich-Epstein, L. B. Tran, O. T. Cox, E. Y. Huang, W. E. Laug, H. Shimada, and M. Millard
Endothelial apoptosis induced by inhibition of integrins {alpha}v{beta}3 and {alpha}v{beta}5 involves ceramide metabolic pathways
Blood,
June 1, 2005;
105(11):
4353 - 4361.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. M. Klein, M. Zheng, A. Ambesi, L. Van De Water, and P. J. McKeown-Longo
Stimulation of extracellular matrix remodeling by the first type III repeat in fibronectin
J. Cell Sci.,
November 15, 2003;
116(22):
4663 - 4674.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. F. Rotundo, T. M. Curtis, M. D. Shah, B. Gao, A. Mastrangelo, S. E. LaFlamme, and T. M. Saba
TNF-alpha disruption of lung endothelial integrity: reduced integrin mediated adhesion to fibronectin
Am J Physiol Lung Cell Mol Physiol,
February 1, 2002;
282(2):
L316 - L329.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Pereira, B. J. Rybarczyk, T. M. Odrljin, D. C. Hocking, J. Sottile, and P. J. Simpson-Haidaris
The incorporation of fibrinogen into extracellular matrix is dependent on active assembly of a fibronectin matrix
J. Cell Sci.,
January 2, 2002;
115(3):
609 - 617.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Resnikoff, T. Brien, P. A. Vincent, R. F. Rotundo, E. Lewis, P. J. McKeown-Longo, and T. M. Saba
Lung matrix incorporation of plasma fibronectin reduces vascular permeability in postsurgical bacteremia
Am J Physiol Lung Cell Mol Physiol,
October 1, 1999;
277(4):
L749 - L759.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. C. Hocking, J. Sottile, T. Reho, R. Fassler, and P. J. McKeown-Longo
Inhibition of Fibronectin Matrix Assembly by the Heparin-binding Domain of Vitronectin
J. Biol. Chem.,
September 17, 1999;
274(38):
27257 - 27264.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Christopher, S. Judge, P. Vincent, P. Higgins, and P. McKeown-Longo
The amino-terminal matrix assembly domain of fibronectin stabilizes cell shape and prevents cell cycle progression
J. Cell Sci.,
January 10, 1999;
112(19):
3225 - 3235.
[Abstract]
[PDF]
|
|
|
|
|
|
|
G. Guadiz, L. A. Sporn, and P. J. Simpson-Haidaris
Thrombin Cleavage-Independent Deposition of Fibrinogen in Extracellular Matrices
Blood,
October 1, 1997;
90(7):
2644 - 2653.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
