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Recombinant human interleukin-3 expands the pool of circulating
hematopoietic progenitor cells in primates--synergism with recombinant
human granulocyte/macrophage colony-stimulating factor
K Geissler, P Valent, P Mayer, E Liehl, W Hinterberger, K Lechner and P Bettelheim
First Department of Medicine, University of Vienna, Austria.
The in vivo effect of recombinant human interleukin-3 (rhIL-3) on
peripheral blood (PB) levels of hematopoietic progenitor cells was studied
in nonhuman primates. Subcutaneous administration of 33 micrograms/kg/d of
rhIL-3 for 11 to 14 days to rhesus monkeys slightly raised leukocyte counts
(twofold) and substantially expanded the pool of circulating stem cells in
the second week of treatment. At the end of rhIL-3 administration, PB
levels of granulocyte/macrophage colony- forming units (CFU-GM) increased
by a mean of 12-fold; burst-forming units-erythroid (BFU-E) by ninefold;
CFU-mix, by 12-fold; and CFU- megakaryocyte (Mk), by 13-fold as compared
with their respective pretreatment values. Subsequent administration of
recombinant human granulocyte/macrophage colony-stimulating factor
(rhGM-CSF; 5.5 micrograms/kg/d for 5 days) to rhIL-3-pretreated animals
further expanded the PB stem cell compartment leading to maximum levels of
CFU- GM that were in average much more increased (63-fold) than CFU-GM
levels under rhIL-3 (14-fold) or rhGM-CSF (12-fold) alone. This hitherto
unknown effect of rhIL-3 on the pool of circulating progenitors,
particularly in synergy with rhGM-CSF, may facilitate harvest of
hematopoietic progenitor cells from PB for stem cell transplantation.
Volume 75,
Issue 12,
pp. 2305-2310,
06/15/1990
Copyright © 1990 by The American Society of Hematology
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