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Interleukin-6 enhances murine megakaryocytopoiesis in serum-free culture
K Koike, T Nakahata, T Kubo, T Kikuchi, M Takagi, A Ishiguro, K Tsuji, K Naganuma, A Okano and Y Akiyama
Department of Pediatrics, Shinshu University School of Medicine, Matsumoto,
Japan.
We investigated the effect of interleukin-6 (IL-6) on murine
megakaryocytopoiesis in a serum-free culture system. The addition of IL- 6
to a culture containing interleukin-3 (IL-3) resulted in a significant
increase in the number of megakaryocyte colonies by bone marrow cells of
normal mice. The megakaryocytic progenitors that survive exposure to
5-fluorouracil (5-FU) exhibited a more significant response to IL-6 and
IL-3. Polyclonal anti-IL-6 antibody neutralized the stimulatory effect of
IL-6 on megakaryocyte colony growth supported by IL-3. Delayed addition
experiments and replating experiments of blast cell colonies showed that
megakaryocytic progenitors are supported by IL-3 in the early stage of the
development but require IL- 6 for their subsequent proliferation and
differentiation. In addition, IL-6 increased the size of megakaryocytes in
granulocyte-macrophage- megakaryocyte colonies. The combination of
granulocyte colony- stimulating factor or granulocyte-macrophage colony
stimulating factor with IL-3 resulted in an increase in the
granulocyte-macrophage colony growth of bone marrow cells of 5-FU-treated
mice or normal mice, respectively, but had little effect on the enhancement
of pure and mixed megakaryocyte colony growth. These results suggest that
IL-6 plays an important role in murine megakaryocytopoiesis.
Volume 75,
Issue 12,
pp. 2286-2291,
06/15/1990
Copyright © 1990 by The American Society of Hematology
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