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Monoclonality in B-lymphoproliferative disorders detected at the DNA level
KJ Trainor, MJ Brisco, CJ Story and AA Morley
Department of Hematology, Flinders Medical Center, Bedford Park, Australia.
A new method was developed for detection of monoclonality at the DNA level
in B-lymphoproliferative disease using the polymerase chain reaction and
consensus primers for the V and J regions of the immunoglobulin gene.
Monoclonality was detected in DNA from 15 of 15 clonal B-lymphoblastoid
cell lines and from 19 of 23 cases of B- lymphocyte neoplasia, but not from
any of 16 normal T-lymphocyte clones, 9 cases of T-lymphocyte neoplasia, 20
samples of polyclonal peripheral blood lymphocytes, and 8 cases of reactive
lymphadenopathy. This method for detection of monoclonality is likely to be
of routine value in diagnosis owing to its simplicity, speed, and
versatility.
Volume 75,
Issue 11,
pp. 2220-2222,
06/01/1990
Copyright © 1990 by The American Society of Hematology
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