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Gova/b alloantigen system on human platelets
JG Kelton, JW Smith, P Horsewood, JR Humbert, CP Hayward and TE Warkentin
Department of Medicine, McMaster Medical Centre, Hamilton, Ontario, Canada.
In this report we describe a platelet alloantigen system that is carried on
a novel platelet protein of 175 Kd. Antisera against the two alleles
(Gova/Govb) were found in two patients who had received large numbers of
platelet transfusions. The anti-Gov alloantibodies could not be detected
using a whole platelet solid phase enzyme immunoassay, or by a platelet
glycoprotein capture enzyme immunoassay using monoclonal antibodies against
glycoproteins Ib/IX, Ia/IIa, and IIb/IIIa. Using radioimmunoprecipitation
techniques, a protein was precipitated that migrated at 175 Kd (reduced).
Under nonreduced conditions, a 150-Kd protein was detected with a minor
component at 175Kd. The detection of the alloantigens was not
activation-dependent. Using immunodepletion studies, we demonstrated that
each alloantiserum recognized an epitope on a discrete population of the
175-Kd platelet protein. Family studies demonstrated that the alloantigens
designated as Gova and Govb were inherited in an autosomal codominant
fashion. The phenotypic frequencies were Gova/Gova, 26%; Gova/Govb, 55%;
Govb/Govb, 19%; giving gene frequencies of 0.532 and 0.468 for Gova and
Govb, respectively (n = 33).
Volume 75,
Issue 11,
pp. 2172-2176,
06/01/1990
Copyright © 1990 by The American Society of Hematology
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